The effect of Notch signal pathway on PM2.5 -induced Muc5ac in Beas-2B cells

Ecotoxicology and Environmental Safety
Ying LiuLiting Zhou

Abstract

Atmospheric pollutants could induced over-expression of Muc5ac, which is a major pathological feature in acute exacerbation of Chronic Obstructive Pulmonary Disease (COPD) and fatal asthma. Notch signaling pathway could promote mucus cell proliferation and mucus secretion. However, the effects of Notch signaling pathway on the airway mucus secretion induced by PM2.5 remain unknown. In this study, we investigated the role of the Notch signaling pathway on Muc5ac by atmospheric PM2.5 in Beas-2B cell. The mRNA and protein levels of the Notch1-4, downstream target gene Hes1 and Muc5ac in the Notch signaling pathway were detected by qPCR and western after Beas-2B cells were exposed to PM2.5 of different concentrations for 12h, 24h, and 48h. The longer the exposure time and the higher the concentration of PM2.5, the lower the survival rate of Beas-2B cells. The expressions of Hes1 and Muc5ac in mRNA and protein were significantly increased after PM2.5 exposure. Correlation analysis indicated that there was a positive correlation between the expression of Muc5ac and Hes1 in mRNA and protein. Atmospheric PM2.5 can induce the express of Muc5ac, the Notch signaling pathway may be involved in the regulation of Muc5ac by Hes1.

References

Oct 6, 1997·The European Respiratory Journal·L D MartinK B Adler
Jan 27, 1999·Seminars in Cell & Developmental Biology·R J Fleming
Nov 6, 2001·American Journal of Respiratory Cell and Molecular Biology·K B Adler, Y Li
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Erik P LillehojK Chul Kim
Jul 18, 2003·The American Journal of Medicine·Laura M KuyperTony R Bai
Feb 6, 2004·British Medical Bulletin·Anoop J Chauhan, Sebastian L Johnston
Jul 19, 2005·Journal of Toxicology and Environmental Health. Part a·Aaron J CohenKirk Smith
Dec 17, 2005·Current Opinion in Pulmonary Medicine·Esteban J Morcillo, Julio Cortijo
Apr 17, 2009·Development·J Sawalla GusehJayaraj Rajagopal
Jul 21, 2009·Biochemical Society Transactions·Jonathan Turner, Carol E Jones
Dec 3, 2010·The New England Journal of Medicine·John V Fahy, Burton F Dickey
Jul 19, 2013·Respiration; International Review of Thoracic Diseases·Hai-Feng Ou-YangZhi-Kui Li
Nov 14, 2013·Air Quality, Atmosphere, & Health·Minjeong ParkHong Yun-Chul
Sep 7, 2014·The Lancet. Respiratory Medicine·Stephen B GordonWilliam J Martin
Sep 23, 2014·JAMA : the Journal of the American Medical Association·Jonathan A PatzAndrew Haines
Oct 18, 2014·The European Respiratory Journal·Anna MölterRaymond Agius
Feb 26, 2015·Environmental Health Perspectives·Bénédicte JacqueminFrancine Kauffmann

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Citations

Jun 27, 2021·Ecotoxicology and Environmental Safety·Liting ZhouLin Ye

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