The effect of oxythioquinox exposure on normal human mammary epithelial cell gene expression: a microarray analysis study

Environmental Health : a Global Access Science Source
Maureen R GwinnAinsley Weston

Abstract

Inter-individual variation in normal human mammary epithelial cells in response to oxythioquinox (OTQ) is reported. Gene expression signatures resulting from chemical exposures are generally created from analysis of exposures in rat, mouse or other genetically similar animal models, limiting information about inter-individual variations. This study focused on the effect of inter-individual variation in gene expression signatures. Gene expression was studied in primary normal human mammary epithelial cells (NHMECs) derived from four women undergoing reduction mammoplasty [Cooperative Human Tissue Network (National Cancer Institute and National Disease Research Interchange)]. Gene transcription in each cell strain was analyzed using high-density oligonucleotide DNA microarrays (HuGeneFL, Affymetrix) and changes in the expression of selected genes were verified by real-time polymerase chain reaction at extended time points (ABI). DNA microarrays were hybridized to materials prepared from total RNA that was collected after OTQ treatment for 15, 60 and 120 min. RNA was harvested from the vehicle control (DMSO) at 120 min. The gene expression profile included all genes altered by at least a signal log ratio (SLR) of +/- 0.6 and p val...Continue Reading

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Citations

Jan 25, 2008·Journal of Toxicology and Environmental Health. Part a·Maureen R Gwinn, Ainsley Weston

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Methods Mentioned

BETA
in vitro transcription
Chip
GTPase
PCR

Software Mentioned

OTQ
MicroDB
GraphPad Prism
Microarray Suite
Affymetrix
MAS
SOM
Data Mining Tool
Microarray
Fluoview

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