The effect of peroxides on the vascular endothelium of isolated pig aorta in vitro

Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie
I KrautschickM Neuenfeld

Abstract

The effect of peroxide on endothelial cells (perfused pig aorta) was examined using an in vitro perfusion model. Hydrogen peroxide was added to the perfusion medium (pig serum together with a buffer solution) which was expected to lead to an increased oxidation of lipids and lipoproteins. Oxidation processes of this type play a decisive role in the pathogenesis and progression of arteriosclerosis. The aim of the present investigation was to demonstrate by introducing hydrogen peroxide (H2O2) in varying concentrations (0.5-1.5%), the destructive impact of peroxides on the endothelium, while these cells are believed to play a key role in the pathogenesis of arteriosclerosis. The extent of endothelial cell impairment was assessed by means of silver staining visualisation of endothelial cell borders as well as light- and scanning-electronmicroscopic investigation. It was discovered that the endothelial cells show increasing impairment after 10 h of perfusion due to the effect of peroxide (hydrogen peroxide).

References

Nov 1, 1975·Atherosclerosis·J B SilkworthW E Stehbens
Aug 1, 1992·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·R Dargel
Mar 1, 1992·Atherosclerosis·R CirilloM Prosdocimi
Nov 1, 1991·Atherosclerosis·J P Cooper, A C Newby
Apr 1, 1990·The Journal of Clinical Investigation·J A BerlinerA M Fogelman
Jun 15, 1989·European Journal of Biochemistry·O CantoniP Cerutti
Jan 1, 1986·Acta Obstetricia Et Gynecologica Scandinavica·K Bjøro, S Stray-Pedersen
Oct 14, 1987·Biochemical and Biophysical Research Communications·A W BernheimerT Uwajima
Apr 1, 1986·Prostaglandins·K BjøroS Stray-Pedersen
Feb 1, 1985·American Journal of Obstetrics and Gynecology·D G GlanceL Myatt
Feb 1, 1986·Journal of Pharmacological Methods·M J CurtisM J Walker
Sep 1, 1985·International Journal of Cardiology·T von ArnimA Maseri
Jun 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·T Morita, R J Bing
May 1, 1983·Molecular and Cellular Endocrinology·P J Hornsby, J F Crivello
May 1, 1984·Prostaglandins, Leukotrienes, and Medicine·B N SettyM J Stuart
Nov 1, 1984·Atherosclerosis·V W Van Hinsbergh
May 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J R HesslerG M Chisolm
Jan 1, 1958·The Journal of Pathology and Bacteriology·J C POOLEH W FLOREY
Apr 1, 1959·The Journal of Pathology and Bacteriology·H W FLOREYG A MEEK

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Citations

Sep 29, 2005·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Koji OgawaYoshio Hatano
Feb 10, 1998·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·J KrugmannI Krautschick
Jul 18, 2014·Nephro-urology Monthly·Santosh KumarSriharsha Ajjoor Shankargowda

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