PMID: 7011884Feb 1, 1981Paper

The effect of pH on 86Rubidium efflux from pancreatic islet cells

Molecular and Cellular Endocrinology
J C Henquin

Abstract

The influence of pH on the K+ permeability of pancreatic islet cells was investigated by measuring 86Rb+ fluxes in isolated rat islets perifused or incubated in the absence of glucose. Acidification of the medium (to pH 6.5), by decreasing the concentration of HCO-3 or by increasing pCO2 at constant HCO-3 reversibly reduced the rate of 86Rb+ efflux from perifused islets. Alkalinization of the medium (to pH 7.8), by decreasing the pCO2, reversibly increased 86Rb+ efflux. Similar changes were recorded upon alteration of the pH in a bicarbonate-free, Hepes-buffered medium with or without calcium. Alteration of the CO2 level at constant external pH - in order to modify internal pH - produced only a small and transient increase in efflux rate when CO2 was lowered, and a decrease in efflux rate when CO2 was raised. NH4Cl reversibly augmented 86Rb+ efflux in the presence and in the absence of HCO-3. At low pH (6.5), 86Rb+ uptake by islet cells was reduced after 10 min (25%) and 30 min (11%), but not after 60 min, of incubation; it was not significantly affected by high pH (7.8). Calcium uptake and insulin release were reduced at low pH and increased at high pH. These results show that the K+ permeability of islet cells is affected by ...Continue Reading

References

Jan 1, 1976·The Journal of General Physiology·W F Boron, P De Weer
Jun 21, 1978·Pflügers Archiv : European journal of physiology·P P van BogaertE E Carmeliet
Jul 25, 1977·Biochemical and Biophysical Research Communications·J C Henquin
Feb 1, 1976·The Journal of General Physiology·C L Schauf, F A Davis
Dec 1, 1974·The Journal of General Physiology·P Shrager
Jun 1, 1973·The Journal of General Physiology·B Hille
Jan 1, 1972·Endocrinology·B HellmanI B Täljedal
Feb 1, 1968·The Journal of General Physiology·B Hille

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Citations

Oct 15, 1984·Experientia·W J MalaisseA Sener
Oct 3, 2006·Biochemical and Biophysical Research Communications·Jocelyn E Manning FoxMichael B Wheeler
Aug 1, 1984·Journal of Endocrinological Investigation·P LebrunW J Malaisse
Jan 1, 1983·The American Journal of Physiology·C S PaceJ S Smith
Mar 1, 1983·The American Journal of Physiology·G T Eddlestone, P M Beigelman
Feb 1, 1986·The American Journal of Physiology·T J BidenC B Wollheim

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