The effect of phospholipase A2 from Crotalus durissus collilineatus on Leishmania (Leishmania) amazonensis infection

Parasitology Research
L F D PasseroM H Toyama

Abstract

In this study, the effect of phospholipase A2 (PLA2) derived from Crotalus durissus collilineatus was evaluated in vitro and in vivo on experimental cutaneous leishmaniasis. The promastigote and amastigote forms treated with PLA2 presented increased growth rate. In vivo studies showed that PLA2-treated Leishmania (Leishmania) amazonensis promastigotes increased the size of lesions in BALB/c mice, and histopathological analysis showed numerous necrotic regions presenting a higher density of polymorphonuclear, mononuclear, and amastigote cells. Additionally, infected macrophages treated with PLA2 were able to generate prostaglandin E2 (PGE2). Cytokine quantification showed that the supernatant from infected macrophages presented moderate and high amounts of IL-2 and IL-10, respectively. However, in PLA2-treated infected macrophages, suppression of IL-2 levels occurred, but not of IL-10 levels. Observation also revealed that both the supernatant and lysate of L. (L.) amazonensis promastigotes exhibited PLA2 activity, which, in the presence of dexamethasone, showed no reduction in their activities; while glucocorticoid maintained the ability of promastigote forms to infect macrophages, which presented values similar to controls. In...Continue Reading

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Citations

Dec 2, 2011·Journal of Parasitology Research·Mário A C Silva-NetoGeorgia C Atella
Sep 17, 2009·Parasitology Research·Luiz Felipe Domingues PasseroMárcia Dalastra Laurenti
Sep 8, 2012·Molecular and Biochemical Parasitology·Mattie C Pawlowic, Kai Zhang
Jan 12, 2016·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Andreia Vieira PereiraBenedito Barraviera
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Dec 1, 2009·Toxicon : Official Journal of the International Society on Toxinology·Alba Fabiola Costa TorresAlice M C Martins
Jan 12, 2010·Parasitology International·Luiz Felipe Domingues PasseroGabriela Santos-Gomes
Mar 10, 2009·Experimental Parasitology·Nordin ZeidnerDonald Champagne
May 24, 2011·Enzyme Research·María Laura BelaunzaránElvira Luisa Durante de Isola
Jan 1, 2013·Molecular and Biochemical Parasitology·María Laura BelaunzaránElvira Luisa Durante de Isola
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Jan 30, 2020·Pathogens·Lucie PodešvováVyacheslav Yurchenko
Mar 3, 2020·Frontiers in Cellular and Infection Microbiology·Anne C S FernandesThaïs Souto-Padrón
Sep 6, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Maria L A C BordonLuiz Felipe D Passero
Jun 8, 2018·Frontiers in Immunology·Adriana Lima VallochiPatricia T Bozza

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