The effect of polyamines on KATP channels in guinea-pig ventricular myocytes

The Journal of Physiology
X W Niu, R W Meech

Abstract

1. The effect of natural polyamines on KATP channels was studied using inside-out patches from guinea-pig ventricular myocytes. 2. At a holding potential of +40 mV, spermine at the intracellular membrane surface reduced the KATP channel open probability (Popen) in a dose-dependent manner. Half-maximal inhibition occurred at 29 microM with a Hill coefficient of 1.2. 3. The effect of spermine on Popen was not greatly influenced by the membrane potential but there appeared to be a small reduction in unitary current amplitude during strong depolarizations. 4. Analysis of KATP single channel kinetics showed that spermine inhibited the channel by decreasing the mean open time and introducing transitions to a long closed state. 5. Spermidine (0.1 mM) was found to have a similar effect to spermine. Putrescine (10 mM) was found to block more effectively at positive membrane potentials. Up to 20 mM arginine had no significant effect on KATP channels. 6. Our results indicate that natural polyamines influence native KATP channel gating in cardiac myocytes.

Citations

Jan 1, 1978·Physiological Reviews·C L Gibbs
Jun 22, 1990·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R Z Kozlowski, M L Ashford
Sep 23, 1985·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D C Ogden, D Colquhoun
Nov 1, 1989·The Journal of General Physiology·J N Weiss, S T Lamp
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S K BallasS B Shohet
Nov 1, 1982·The American Journal of Physiology·A E Pegg, P P McCann
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M SchindlerM P Sheetz
May 1, 1981·Journal of Neurochemistry·R J Harman, G G Shaw
Apr 1, 1993·Trends in Neurosciences·R H ScottA C Dolphin
Oct 1, 1994·The Journal of General Physiology·N DeutschJ N Weiss
Jun 1, 1994·The Journal of Membrane Biology·T Weiger, A Hermann
Mar 1, 1994·The European Journal of Neuroscience·H Drouin, A Hermann
Mar 1, 1993·The Journal of Physiology·M D HermanT Narahashi
Jan 1, 1996·Circulation Research·C G NicholsA N Lopatin
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·S ShyngC G Nichols
Feb 28, 1997·The Journal of Biological Chemistry·Z Fan, J C Makielski
May 1, 1997·Neuron·J P ClementJ Bryan
Aug 1, 1997·The Journal of General Physiology·S ShyngC G Nichols

Related Concepts

Biogenic Polyamines
Arginine hydrochloride
Potassium Channel Blockers
Membrane
Natural Products
Left Ventricular Structure
Resting Potentials
Putrescine
Myocardium
Spermine

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.