The effect of polyamines on KATP channels in guinea-pig ventricular myocytes

The Journal of Physiology
X W Niu, R W Meech


1. The effect of natural polyamines on KATP channels was studied using inside-out patches from guinea-pig ventricular myocytes. 2. At a holding potential of +40 mV, spermine at the intracellular membrane surface reduced the KATP channel open probability (Popen) in a dose-dependent manner. Half-maximal inhibition occurred at 29 microM with a Hill coefficient of 1.2. 3. The effect of spermine on Popen was not greatly influenced by the membrane potential but there appeared to be a small reduction in unitary current amplitude during strong depolarizations. 4. Analysis of KATP single channel kinetics showed that spermine inhibited the channel by decreasing the mean open time and introducing transitions to a long closed state. 5. Spermidine (0.1 mM) was found to have a similar effect to spermine. Putrescine (10 mM) was found to block more effectively at positive membrane potentials. Up to 20 mM arginine had no significant effect on KATP channels. 6. Our results indicate that natural polyamines influence native KATP channel gating in cardiac myocytes.


Jan 1, 1978·Physiological Reviews·C L Gibbs
Jun 22, 1990·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R Z Kozlowski, M L Ashford
Sep 23, 1985·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D C Ogden, D Colquhoun
Nov 1, 1989·The Journal of General Physiology·J N Weiss, S T Lamp
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S K BallasS B Shohet
Nov 1, 1982·The American Journal of Physiology·A E Pegg, P P McCann
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M SchindlerM P Sheetz
May 1, 1981·Journal of Neurochemistry·R J Harman, G G Shaw
Apr 1, 1993·Trends in Neurosciences·R H ScottA C Dolphin
Oct 1, 1994·The Journal of General Physiology·N DeutschJ N Weiss
Jun 1, 1994·The Journal of Membrane Biology·T Weiger, A Hermann
Mar 1, 1994·The European Journal of Neuroscience·H Drouin, A Hermann
Mar 1, 1993·The Journal of Physiology·M D HermanT Narahashi
Jan 1, 1996·Circulation Research·C G NicholsA N Lopatin
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·S ShyngC G Nichols
Feb 28, 1997·The Journal of Biological Chemistry·Z Fan, J C Makielski
May 1, 1997·Neuron·J P ClementJ Bryan
Aug 1, 1997·The Journal of General Physiology·S ShyngC G Nichols

Related Concepts

Biogenic Polyamines
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