PMID: 999868Dec 21, 1976

The effect of poly(ethyleneglycol) esters on the partition of proteins and fragmented membranes in aqueous biphasic systems

Biochimica Et Biophysica Acta
G Johansson

Abstract

The hydroxyl groups of poly(ethyleneglycol) have been esterified (partly) with a number of carboxylic acids. When these esters are included in dextranpoly(ethyleneglycol)-water biphasic systems the partitions of proteins and membranes between the two phases (and the interface) are in some cases strongly affected. The affinity of serum albumin for the poly(ethyleneglycol)-rich phase is strongly increased when the fatty acid group consists of more than 10 carbon atoms. The partition also depends on the number of double bonds in the fatty acid. A corresponding relationship is found for membranes from spinach chloroplasts. The partitions of ovalbumin, lysozyme (EC 3.2.1.17) and ribonuclease (EC 3.1.4.22) are not influenced by the fatty acid esters. Esters of dibasic carboxylic acids show a minute but marked effect on the partition of proteins in general while malate and tartrate esters affect strongly the partition of chloroplast membranes. The partitions of both proteins and membranes are influenced by poly(ethyleneglycol) deoxycholate. Experiments with malate dehydrogenase (EC 1.1.1.37), lactate dehydrogenase (EC 1.1.1.27), fumarase (EC 4.2.1.2), enolase (EC 4.2.1.11) and glutamate-ocaloacetate transaminase (EC 2.6.1.1) show that...Continue Reading

Citations

Aug 20, 1990·Biotechnology and Bioengineering·C LaMarcaP Dhurjati
Sep 15, 1990·International Journal of Cancer. Journal International Du Cancer·M WakselmanC Chany
Jun 1, 1986·Analytical Biochemistry·H Walter, G Johansson
May 1, 1976·Experimental Neurology·G ZanchinA Lajtha
Aug 15, 1982·Biochemical Pharmacology·C WheelerD Kessel
Nov 15, 1981·Biochemical Pharmacology·T H Chou, D Kessel
Aug 5, 1989·Biotechnology and Bioengineering·J N BaskirU W Suter
Apr 5, 1983·European Journal of Biochemistry·G JohanssonP A Albertsson
Jan 15, 1979·European Journal of Biochemistry·V Shanbhag, G Johansson
Jan 1, 1980·Scandinavian Journal of Infectious Diseases. Supplementum
Apr 1, 1978·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·K E MagnussonL Edebo

Related Concepts

Esters
Membranes, Artificial
Vigilon
Structure-Activity Relationship
Viscosity

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