The effect of polymer size and charge of molecules on permeation through synovial membrane and accumulation in hyaline articular cartilage

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
B SternerC M Lehr

Abstract

The treatment of joint related diseases often involves direct intra-articular injections. For rational development of novel delivery systems with extended residence time in the joint, detailed understanding of transport and retention phenomena within the joint is mandatory. This work presents a systematic study on the in vitro permeation, penetration and accumulation of model polymers with differing charges and molecular weights in bovine joint tissue. Permeation experiments with bovine synovial membrane were performed with PEG polymers (6-200 kDa) and methylene blue in customized diffusion chambers. For polyethylene glycol, 2-fold (PEG 6 kDa), 3-fold (PEG 10 kDa) and 13-fold (PEG 35 kDa) retention by the synovial membrane in reference to the small molecule methylene blue was demonstrated. No PEG 200 kDa was found in the acceptor in detectable amounts after 48 h. This showed the potential for a distinct extension of joint residence times by increasing molecular weights. In addition, experiments with bovine cartilage tissue were conducted. The ability for positively charged, high molecular weight chitosans and HEMA-Co-TMAP (HCT) polymers (up to 233 kDa) to distribute throughout the entire cartilage matrix was demonstrated. In co...Continue Reading

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Citations

Sep 7, 2018·Experimental and Therapeutic Medicine·Numan KaraarslanOzkan Ates
Feb 17, 2017·Nature Reviews. Rheumatology·Ambika G Bajpayee, Alan J Grodzinsky
Aug 29, 2020·Bioelectricity·Shreedevi Kumar, Blanka Sharma
Jan 27, 2021·Annals of Biomedical Engineering·Young GuangLori A Setton
Feb 11, 2021·Pharmaceuticals·Oliver McClurgLinda Troeberg
Mar 12, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tobias SiefenAlf Lamprecht
Apr 22, 2021·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sandra Barbosa Neder AgostiniFlávia Chiva Carvalho

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