PMID: 15369242Sep 17, 2004Paper

The effect of posthypoxic hypothermia for lactate dehydrogenase activity in brain cortex and blood serum of 14-days-old rats

Ceská gynekologie
L SmídováA Dohnalová

Abstract

The effect of various degrees of hypobaric hypoxia and consequent hypothermia on lactate dehydrogenase (E.C.1.1.1.27) activity in blood serum and brain cortex in 14 day-old rats was investigated. Experimental study. Institute of Physiology, 1st Med. Faculty, Charles University, Prague. 14 day-old rats (Wistar of our own breed) were exposed to mild hypobaric hypoxia (corresponding to altitude of 7000 m, pO2 = 8.6 kPa, BP = 41.2 kPa, lasting 20 min) or strong hypobaric hypoxia (corresponding to altitude of 9000 m, pO2 = 6.4 kPa, BP = 30.7 kPa, lasting 30 min). Just after hypoxic stress the animals were killed by decapitation and on cooled block the grey cortical matter was removed. Blood serum samples as well as brain cortex homogenates were immediately incubated in three variously tempered media (38 degrees Celsius = control values, 30 degrees Celsius and 22 degrees Celsius = hypothermic conditions). The incubation was over (30 min.), and the lactate dehydrogenase (LDH) activity was followed using the Lachema test. The LDH activity was influenced by mild hypobaric hypoxia (7000 m) neither in blood serum nor in brain cortex. Also the values of LDH activities registered in normo or in hypothermic conditions were not different as c...Continue Reading

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