PMID: 9550216Apr 29, 1998Paper

The effect of praziquantel treatment on glutathione concentration in Schistosoma mansoni

Parasitology
F RibeiroJ R Kusel

Abstract

A fluorescent dye monochlorobimane (MCB) that binds glutathione (GSH) was used as a tool for measuring the concentration of GSH in skin and mechanically-transformed schistosomula. The specificity of MCB binding to GSH was confirmed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The MCB binding to GSH is an energy-dependent process since no labelling could be seen at low temperature. When 24-h-old schistosomula were depleted of GSH by buthionine sulfoximine (a specific inhibitor of GSH synthesis) for 18 h, a significant decrease (P < 0.001) in fluorescence was observed. PZQ treatment of the schistosomula after first labelling the parasites with MCB did not greatly affect MCB binding to GSH. However, when the 24-h-old schistosomula were first PZQ treated and afterwards labelled with MCB, the pattern of labelling was identical to that of those of the non-labelled parasites. When 24-h-old schistosomula were first PZQ treated, washed and labelled in the presence of 1 mM GSH, the level of fluorescence was recovered. These results suggest that PZQ depletes GSH from schistosomula, and may render them susceptible to the host's immune system.

Citations

Mar 4, 2009·Parasitology Research·Rashad Abdul-GhaniAzza Hassan
Jul 18, 2001·The Journal of Eukaryotic Microbiology·M L KentC Xiao
Nov 5, 2002·Annual Review of Entomology·Hilary Hurd
Dec 25, 2013·PLoS Neglected Tropical Diseases·Regina CoeliGuilherme Oliveira
Oct 2, 2012·Molecular and Biochemical Parasitology·Jarrett Hines-KayCharles Cunningham
Dec 23, 2008·Molecular and Biochemical Parasitology·Anthony D AragonCharles Cunningham
Jul 12, 2008·Acta Tropica·N Katz, P M Z Coelho
May 4, 2011·Memórias do Instituto Oswaldo Cruz·Flávia F B CoutoAna Carolina A Mattos
Sep 14, 2004·Revista Do Instituto De Medicina Tropical De São Paulo·Fabio RibeiroPaulo Marcos Zech Coelho
Jun 14, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Iman F Abou-El-Naga

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