The effect of prior in vitro exposure of donor cells to trophic factors in neurotransplantation

Experimental Neurology
X L ChenM Gupta

Abstract

The ability of PC12 cells to regenerate processes is substantially enhanced in vitro if they have been previously exposed to nerve growth factor (NGF-primed), compared to cells that have not been exposed (NGF-naive). These studies were carried out to determine if the enhanced neuritogenic ability of NGF-primed cells is retained following transplantation. NGF-naive or NGF-primed PC12 cells were transplanted into the striatum of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice and allowed to survive for 2 weeks. Mice were given daily injections of cyclosporin A (CyA) to prevent anti-species graft rejection. The transplanted PC12 cells were visualized by tyrosine hydroxylase immunoreactivity. The NGF-naive transplanted cells formed dense clusters and large tumor masses in more than half the animals. Only a few of the naive PC12 cells had short processes. In contrast, many of the transplanted NGF-primed PC12 cells had processes. Furthermore, fewer of the animals transplanted with primed cells produced tumor masses in the striatum compared to animals that received NGF-naive cells. Transplantation of NGF-naive PC12 cells leads to a significant increase in the number of dopaminergic neurons in the host substantia nigra...Continue Reading

Citations

Jul 31, 1998·Reviews in the Neurosciences·A C GranholmR T Bartus
Jun 22, 2006·Neurotoxicity Research·J Garcia de YebenesM A Mena
Jul 2, 2003·Neurotoxicity Research·Justo Garcia De YébenesMaria Angeles Mena
Jun 1, 1997·Transplantation Proceedings·P R SanbergD F Cameron

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