The effect of prostaglandin E2 on costochondral chondrocyte differentiation is mediated by cyclic adenosine 3',5'-monophosphate and protein kinase C

Endocrinology
Z SchwartzB D Boyan

Abstract

Recent studies indicate that vitamin D metabolites exert rapid effects on growth plate chondrocytes via changes in PG production and protein kinase C (PKC) activity. This suggests that these two products of vitamin D action may be interrelated. To test this hypothesis, we examined the effect of PGE2 on rat costochondral resting zone and growth zone cartilage cells and determined whether the effects of PGE2 are mediated by changes in the level of cAMP and/or PKC activity, whether there is a relationship between cAMP production and PKC activity, and whether cell maturation-specific effects are involved. Confluent, fourth passage resting zone and growth zone cartilage cell cultures were incubated in DMEM containing 10% FBS, 50 microg/ml vitamin C, and 1% antibiotics. The PGE2 concentration was varied from 0.007-15 ng/ml. Low concentrations of PGE2 caused a dose-dependent increase in cell number and [3H]thymidine incorporation and stimulated alkaline phosphatase specific activity. These effects were comparable in resting zone and growth zone cartilage cells at the same PGE2 concentrations. At higher concentrations, PGE2 caused a general increase in the synthesis of collagenase-digestible protein and noncollagenase-digestible protei...Continue Reading

Citations

Jan 1, 2009·Experimental & Molecular Medicine·Won Kil LeeSong Ja Kim
Oct 24, 2009·Der Orthopäde·C BrochhausenC J Kirkpatrick
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B D BoyanZ Schwartz
Jan 2, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J SelaZ Schwartz
Mar 7, 2014·Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders·Ross A Hauser, Ingrid Schaefer Sprague
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Rebecca K Studer, Constance R Chu
Oct 20, 2018·Journal of Cellular Biochemistry·Tingshuai Zhang, Yongchang Yao
Nov 19, 2014·Endocrinology·Michele R Hutchison, Perrin C White
Mar 16, 2001·Clinical Orthopaedics and Related Research·H H GuerkovB D Boyan
Mar 20, 2001·Journal of Biomedical Materials Research·B D BoyanZ Schwartz
Oct 14, 2004·Clinical Orthopaedics and Related Research·Mary B Goldring, Francis Berenbaum

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