PMID: 6090838Oct 1, 1984

The effect of pyridoxal-5-phosphate on the inhibition of platelet aggregation and adenosine-3'-5'-cyclic monophosphate accumulation in human platelets

Life Sciences
M ZahaviV V Kakkar


Platelet adenosine-3'-5'-cyclic monophosphate (c-AMP) was determined in platelet rich plasma and washed platelets by a modification of the c-AMP protein binding assay. Studies were performed in the presence and absence of platelet aggregation inducers (adenosine diphosphate and thrombin) and inhibitors (pyridoxal-5-phosphate (PALP), prostaglandin E1 (PGE1), theophylline and papaverine). At 70% inhibitory concentration of platelet aggregation (PA) induced by papaverine or theophylline, a small but significant increase in platelet c-AMP level was found. With PALP however the inhibition of PA was not associated with a significant increase in the c-AMP level. PALP or theophylline potentiated greatly both the inhibition of PA and c-AMP accumulation induced by PGE1. Yet PALP potentiated the inhibition of PA induced by theophylline without increasing platelet c-AMP level. Our results indicate that the inhibition of PA by PALP is not mediated by c-AMP accumulation. However in the presence of PGE1, the increment in PA inhibition, is mediated by further indirect activation of the adenyl-cyclase.


Nov 9, 2012·Amino Acids·Peter SchemmerRonald G Thurman
Aug 20, 2019·Platelets·Gennadi Kobzar
Apr 30, 2020·Journal of Clinical Medicine·Brigitte TardyClaire Pouplard


Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G A Rodan, M B Feinstein
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H B PollardN R Shulman
Mar 1, 1977·Prostaglandins·R R GormanO V Miller
May 28, 1979·Biochemical and Biophysical Research Communications·K Subbarao, V V Kakkar
Sep 1, 1977·Circulation·N H Silverman, C L Roge
Jan 1, 1967·Annual Review of Biochemistry·P Fasella
Oct 23, 2008·Chemistry : a European Journal·Moritz von Hopffgarten, Gernot Frenking

Related Concepts

Adenosine Diphosphate Measurement
Protein Binding
Platelet Aggregation Measurement
F2 gene
ALPP gene
Pyridoxal Phosphate

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