Abstract
The dithiothreitol-dependent vitamin K 2,3-epoxide (vitamin KO) reductase activity was assayed in rat liver, kidney and testis microsomes. Rat kidney and testis showed vitamin KO reductase activity. The activity was about one tenth of the activity present in liver microsomes. The effect of in vivo S-warfarin was investigated after single doses, i.e. 0.2, 0.4 and 1 mg/kg, and after its chronic administration, i.e. 4.8 micrograms/kg/hr for 3 days. At 20 hr following the acute warfarin administration vitamin KO reductase in liver microsomes was depressed in a dose-dependent way, 50, 30 and 20% of control activity. Vitamin KO reductase in testis was not affected, and in kidney reductase activity was only reduced after the highest warfarin dose, 40% of control activity. Following chronic administration of warfarin, vitamin KO reductase activity was reduced in liver as well as in kidney and testis microsomes, 15-20, 40 and 60% of control activity in liver, kidney and testis, respectively. Blood clotting activity was about 14% of normal (thrombotest). Vitamin KO reductase activity in tissue microsomes was inhibited by warfarin added in vitro. Tissue and microsomal warfarin concentration were assayed. Following the acute administration...Continue Reading
References
Aug 23, 1976·Biochemical and Biophysical Research Communications·P V HauschkaP M Gallop
Nov 14, 1979·Biochemical and Biophysical Research Communications·R J LevyP Gallop
Mar 7, 1979·Biochimica Et Biophysica Acta·P A FriedmanJ K Wallace
Apr 18, 1978·Biochemistry·D S WhitlonJ W Suttie
Mar 1, 1986·Journal of Pharmaceutical Sciences·M J DaemenH H Thijssen
Nov 1, 1985·The Journal of Clinical Investigation·R Wallin, L F Martin
Mar 1, 1983·Biochemistry·S D Buchthal, R G Bell
Dec 1, 1983·The Journal of Pharmacy and Pharmacology·H H ThijssenM J Reijnders
Jan 1, 1984·Molecular and Cellular Biochemistry·C Vermeer
Apr 1, 1984·The Journal of Clinical Investigation·J B LianJ Glowacki
Nov 22, 1983·Biochemistry·M J FascoP A Friedman
Aug 12, 1983·Biochemical and Biophysical Research Communications·M C RoncaglioniC Vermeer
Feb 1, 1984·Archives of Biochemistry and Biophysics·E F HildebrandtJ W Suttie
May 13, 1983·Journal of Chromatography·H H ThijssenM J Reijnders
Jan 1, 1980·The American Journal of Medicine·J G HallK M Wilson
Jun 26, 1980·The New England Journal of Medicine·P M GallopP V Hauschka
Nov 8, 1982·FEBS Letters·C VermeerM Daemen
Dec 31, 1980·Biochemical and Biophysical Research Communications·M J Fasco, L M Principe
Citations
Mar 1, 1988·Biochemical Pharmacology·S E Hazelett, P C Preusch
Nov 1, 1990·Biochemical Pharmacology·T M Misenheimer, J W Suttie
Nov 6, 1991·Biochemical Pharmacology·H H Thijssen, L G Baars
Jul 20, 2000·Chemosphere·C A BouwmanM Van den Berg
Nov 14, 1996·Biochimica Et Biophysica Acta·J E RondenC Vermeer
Mar 7, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Motohiro HoshinoKiyoshi Sugiyama
Oct 22, 2014·The American Journal of the Medical Sciences·Malcolm D KearnsVin Tangpricha
Sep 10, 2013·Environmental Toxicology and Chemistry·Barnett A RattnerJohn J Johnston
Mar 5, 2013·Journal of Thrombosis and Haemostasis : JTH·A FreginJ Oldenburg
Aug 15, 1989·Biochemical Pharmacology·T MatsubaraT Yoshizaki
Apr 1, 1989·Biochemical Pharmacology·H H Thijssen, L G Baars
Jul 22, 2008·Pest Management Science·Bastiaan G MeerburgAize Kijlstra
Jul 1, 1995·The Journal of Urology·M GrégoireW C DeWolf
Aug 10, 2000·The Journal of Biological Chemistry·V P MutucumaranaS M Wu
Sep 12, 2003·The Journal of Biological Chemistry·Vasantha P MutucumaranaDarrel W Stafford
Nov 1, 1988·British Journal of Pharmacology·H H ThijssenH T Vervoort-Peters
Jan 25, 2018·Laboratory Medicine·Sierra C SimmonsLance A Williams
Apr 1, 1988·The Journal of Pharmacy and Pharmacology·M J DaemenH H Thijssen
Feb 3, 2021·Molecular and Cellular Biology·Sachiko ShibaSatoshi Inoue
Sep 1, 1987·Biochemical Pharmacology·H H Thijssen
Feb 6, 2007·Bioorganic & Medicinal Chemistry·Markus Gebauer