The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells

PloS One
Miina OjansivuSusanna Miettinen

Abstract

Despite the good performance of silicate bioactive glasses in bone regeneration, there is considerable potential to enhance their properties by chemical modifications. In this study, S53P4-based borosilicate glasses were synthesized and their dissolution profile was studied in simulated body fluid by assessing pH change, ion release and conversion to hydroxyapatite. The viability, proliferation, attachment, osteogenesis and endothelial marker expression of human adipose stem cells (hASCs) was evaluated upon direct culture on glass discs and in the extract medium. This is the first study evaluating cell behavior in response to borosilicate glasses based on S53P4 (commercially available as BonAlive®). Replacing silicate with borate in S53P4 increased the glass reactivity. Despite the good viability of hASCs under all conditions, direct culture of cells on borosilicate discs and in undiluted extract medium reduced cell proliferation. This was accompanied with changes in cell morphology. Regarding osteogenic commitment, alkaline phosphatase activity was significantly reduced by the borosilicate glass discs and extracts, whereas the expression of osteogenic markers RUNX2a, OSTERIX, DLX5 and OSTEOPONTIN was upregulated. There was als...Continue Reading

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Citations

May 16, 2019·Journal of Biomedical Materials Research. Part a·Ayush MishraJonathan Massera
Sep 1, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kaisa VuornosSusanna Miettinen
Jun 12, 2020·Biological Trace Element Research·Ahmet Engin PazarçevirenZafer Evis
Mar 25, 2020·Journal of Materials Science. Materials in Medicine·Nathan J ThyparambilJulie A Semon
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Hyeree ParkShowan N Nazhat
Nov 26, 2019·Materials Science & Engineering. C, Materials for Biological Applications·A HouaouiM Boissière

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Methods Mentioned

BETA
X-ray
flow cytometry
Assay

Software Mentioned

BonAlive
SPSS Statistics

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