The effect of sample preparation and storage on maternal triple-marker screening

Obstetrics and Gynecology
M E LantzM C Williams

Abstract

To evaluate the effect of different sample collection, storage, and preparation techniques on serum alpha-fetoprotein (AFP), beta-hCG, and unconjugated estriol (E3) concentrations. A solution containing known concentrations of AFP, hCG, and unconjugated E3 was diluted in blood samples obtained from seven healthy male volunteers. Serum from each blood sample was removed immediately, and either assayed or frozen at -70C. Portions of the remaining blood were handled as follows: centrifuged and refrigerated, centrifuged and left at room temperature, not centrifuged and refrigerated, or not centrifuged and left at room temperature. Serum was removed from these samples for triple-marker analysis at 24, 48, 96, and 168 hours after the initial sample collection. Immediate freezing of serum and subsequent thawing resulted in a significant increase in beta-hCG and unconjugated E3 levels, but no change in AFP levels. There was a significant effect over time on AFP, hCG, and unconjugated E3 concentrations. The change in AFP levels was influenced by centrifugation status, whereas all three analytes were influenced by refrigeration status. Different sample collection, storage, and preparation techniques may affect maternal triple-marker scre...Continue Reading

References

Nov 8, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·K H MoscropD Burnett
Dec 1, 1993·American Journal of Obstetrics and Gynecology·G E PalomakiJ H Eckfeldt

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Citations

Jan 19, 2011·Intensive Care Medicine·Philippe R BauerOgnjen Gajic
Jun 10, 1998·Obstetrical & Gynecological Survey·A Conde-Agudelo, A C Kafury-Goeta
Oct 8, 2015·Clinical Hemorheology and Microcirculation·Ferenc KissNorbert Nemeth
Jul 12, 2011·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Daljit Singh SahotaTze Kin Lau
Jul 29, 1998·Annals of Clinical Biochemistry·C M Sturgeon, E J McAllister

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