The Effect of Sea Buckthorn (Hippophae rhamnoides L.) Seed Oil on UV-Induced Changes in Lipid Metabolism of Human Skin Cells

Antioxidants
Agnieszka GęgotekElzbieta Skrzydlewska

Abstract

Lipids and proteins of skin cells are the most exposed to harmful ultraviolet (UV) radiation contained in sunlight. There is a growing need for natural compounds that will protect these sensitive molecules from damage, without harmful side effects. The aim of this study was to investigate the effect of sea buckthorn seed oil on the redox balance and lipid metabolism in UV irradiated cells formed different skin layers to examine whether it had a protective effect. Human keratinocytes and fibroblasts were subjected to UVA (ultraviolet type A; 30 J/cm² and 20 J/cm²) or UVB (ultraviolet type B; 60 mJ/cm² and 200 mJ/cm², respectively) radiation and treated with sea buckthorn seed oil (500 ng/mL), and the redox activity was estimated by reactive oxygen species (ROS) generation and enzymatic/non-enzymatic antioxidants activity/level (using electron spin resonance (ESR), high-performance liquid chromatography (HPLC), and spectrophotometry). Lipid metabolism was measured by the level of fatty acids, lipid peroxidation products, endocannabinoids and phospholipase A2 activity (GC/MS (gas chromatography/mass spectrometry), LC/MS (liquid chromatography/mass spectrometry), and spectrophotometry). Also, transcription factor Nrf2 (nuclear eryt...Continue Reading

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Citations

Mar 20, 2020·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Denisa SkarupovaAlena Rajnochova Svobodova
Nov 9, 2018·Antioxidants·Neven Zarkovic
May 30, 2020·Food Research International·Zuzana CiesarováZuzana Burčová
Mar 31, 2021·Critical Reviews in Food Science and Nutrition·Kewen WangXiaojun Liao
Oct 6, 2020·Journal of Ethnopharmacology·Swati PundirPoonam Negi
Jun 3, 2021·Antioxidants·Kamila LaskośIlona Mieczysława Czyczyło-Mysza
Aug 3, 2021·International Journal of Radiation Biology·Alena RyšaváAlena Rajnochová Svobodová
Nov 10, 2021·Molecular Medicine Reports·Joong Suk JeonYoung-Whan Choi

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Methods Mentioned

BETA
ELISA
electrophoresis

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