PMID: 20635040Jul 17, 2010Paper

The effect of serum concentration and culture time on the biologic activity of human periodontal ligament cells

Shanghai kou qiang yi xue = Shanghai journal of stomatology
Ling-ling EDong-sheng Wang

Abstract

To investigate the effect of serum concentration and culture time on the biologic activity of human periodontal ligament cells (HPDLCs). The proliferation (1,4,7,10,14,21d), the protein synthesis, the ALP activity, the collagen synthesis, the calcium intake and the osteocalcin secretion (4,7d) of the fourth passage (P4) HPDLCs treated with 10 mL/L or 50 mL/L neonatal bovine serum (NBS) were examined using MTT method, BCA protein concentration, ALP activity,hydroxyproline and Roche Diagnostics cobas calcium content reagent kits and radio-immunity assay. The data were subjected to two-way analysis of variance and t test using SPSS13.0 software package. 50 mL/L NBS could significantly increase the proliferation (1,4,7,10,14,21d) and the protein synthesis (4,7d) of the P4 HPDLCs. 50 mL/L NBS could significantly inhibit their ALP activity, the calcium intake (4,7d) and the osteocalcin secretion (7d). But it had no effect on the collagen synthesis. During cell culture, the two serum concentrations and the culture time only had interaction on the proliferation, ALP activity and osteocalcin secretion of the P4 HPDLCs. The serum concentration and culture time could affect the biologic activity of HPDLCs.

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