Nov 22, 2013

The effect of sevoflurane on developing A/J strain mouse embryos using a whole-embryo culture system--the incidence of cleft lip in culture embryos

In Vitro Cellular & Developmental Biology. Animal
Morimasa YamadaN Natsume

Abstract

A/J strain mice have a high spontaneous incidence of cleft lip (ICL) and/or palate. The primary palate-related effects of sevoflurane on developing A/J strain mouse embryos (embryos) were studied using a whole-embryo culture (WEC) system. This system could separate the direct effects of sevoflurane from those that are maternally mediated. A total of 205 10.5-d embryos were cultured for 24 h in either a control group (control gas: 95% O2 and 5% CO2) or sevoflurane-administered groups (1/4, 1/2, and 1 minimum alveolar concentration (MAC) with control gas) for 8 h. After 16 h, 11.5-d culture embryos were examined in terms of crown-rump length, number of somites, and protein content. Crown-rump length in the 1 MAC was significantly shorter than in the control group (p < 0.05). Protein content in the 1/2 MAC (p < 0.05) and 1 MAC (p < 0.001) was significantly lower than in the control group. The ICL showed no significant differences between each group. (The ICL rose with an increase in the sevoflurane concentration, but this was not significant). The positive findings in this study indicate that a WEC system is useful for studying the mechanisms of ICL (teratogenicity) associated with sevoflurane.

  • References21
  • Citations2

Citations

Mentioned in this Paper

Embryo
Methyl Ethers
Sevoflurane
Alveolar
Anesthetic Gases
Ultane
Teratogens
Somites
Cleft Lip
Culture of Embryo

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Related Papers

Research Communications in Chemical Pathology and Pharmacology
A YokoyamaH Kitagawa
The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association
Gregory Stylianos Antonarakis, Stavros Kiliaridis
© 2020 Meta ULC. All rights reserved