The effect of sevoflurane on glutamate release and uptake in rat cerebrocortical presynaptic terminals

Acta Anaesthesiologica Scandinavica
Morten L VinjeJon Berg-Johnsen

Abstract

Volatile anaesthetics exert their effect in the brain mainly by reducing synaptic excitability. Isoflurane abates excitation by reducing the release and increasing the uptake of transmitter glutamate into the presynaptic terminal. The exact molecular mechanisms exerting these effects, however, are not clear. Voltage-gated calcium channels have been proposed as the pharmacological target. The present study examines the effect of sevoflurane on synaptic glutamate release and free cytosolic calcium and the effect on high- and low-affinity uptake of L-glutamate using isolated presynaptic terminals prepared from rat cerebral cortex. Released glutamate was measured fluorometrically in a spectrophotometer as the fluorescence of NADPH and calcium as the fluorescence of fura-2. 4-aminopyridine was used to induce membrane depolarization. Glutamate uptake was measured in a series of different concentrations of L-glutamate corresponding to the high- and the low- affinity uptake systems adding a fixed concentration og radiolabelled glutamate. The labelling was measured by counting disintegrations per min in a beta-scintillation counter. Sevoflurane reduced the calcium-dependent glutamate release in a dose-dependent manner as sevoflurane 1.5...Continue Reading

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Citations

Jul 12, 2008·International Anesthesiology Clinics·Christa J Van DortRalph Lydic
Mar 12, 2010·Tidsskrift for den Norske lægeforening : tidsskrift for praktisk medicin, ny række·Knut Arvid KirkebøenJohan Raeder
Jan 6, 2006·The International Journal of Neuroscience·Marie-Françoise RitzAminadav Mendelowitsch
Apr 9, 2009·Expert Opinion on Pharmacotherapy·Fabrice Michel, Jean-Michel Constantin
Nov 27, 2008·Acta Anaesthesiologica Scandinavica·Ravi BainsJ Berg-Johnsen
Sep 26, 2008·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Maolin QiuR Todd Constable
Apr 29, 2006·Acta Anaesthesiologica Scandinavica·R BainsM L Vinje
May 4, 2004·Annales Françaises D'anesthèsie Et De Rèanimation·C De DeyneP Ravussin
Apr 23, 2004·Acta Anaesthesiologica Scandinavica·M C MoeJ Berg-Johnsen
Aug 13, 2002·Journal of Neurosurgical Anesthesiology·Morten C MoeMorten L Vinje
Dec 2, 2020·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Yong ZuoZhenhua Shi

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