PMID: 2505851Sep 11, 1989Paper

The effect of shear stress on the uptake and metabolism of arachidonic acid by human endothelial cells

Biochimica Et Biophysica Acta
M U NollertL V McIntire

Abstract

The uptake and metabolism of arachidonic acid (AA) by human umbilical vein endothelial cells was studied for cells in stationary culture and for cells exposed to physiological levels of shear stress. For cells grown in stationary culture, the initial incorporation of arachidonic acid was primarily into diacylglycerol and phospholipids. Cells exposed to flow incorporated labeled arachidonic acid at a similar rate as cells maintained in stationary culture; however, the distribution of the label was altered by flow. The incorporation of arachidonic acid into diacylglycerol and phosphatidylinositol was increased in cells exposed to flow. The largest increase occurred for cells exposed to arterial levels of shear stress for the shortest time period studied, 0.5 h. Prostacyclin (PGI2) and PGF2 alpha were the principal arachidonic acid metabolites formed. Shear stress-stimulated cells preferentially produced PGI2 relative to other eicosanoid products. The initiation of flow caused a burst of AA metabolism which was highly specific for PGI2. This might represent an increase in the turnover of phosphatidylinositol-bound arachidonic acid which is specifically converted to PGI2 as a result of flow-induced membrane stresses.

References

Nov 1, 1978·The Journal of Clinical Investigation·B B WekslerE A Jaffe
Feb 26, 1988·Science·D L Alkon, H Rasmussen
Mar 1, 1988·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H HalldórssonG Thorgeirsson
Sep 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J AndoA Kamiya
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Jan 19, 1988·Biochimica Et Biophysica Acta·S RajagopalanK K Wu
Sep 12, 1984·Biochimica Et Biophysica Acta·M SivarajanC Manner
May 1, 1980·The Journal of Clinical Investigation·A A SpectorJ B Smith
Dec 15, 1981·Biochemical and Biophysical Research Communications·J M ThomasL Douste-Blazy
Apr 15, 1982·The Biochemical Journal·R F Irvine
Oct 13, 2001·The Journal of Organic Chemistry·M M Greenberg, J D Kahl
Oct 5, 1988·Biotechnology and Bioengineering·J A FrangosS G Eskin

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Citations

Dec 19, 2000·Biochemical and Biophysical Research Communications·M IwamotoY Nakaoka
May 1, 1997·In Vitro Cellular & Developmental Biology. Animal·K M FrancisG F Spaulding
Oct 31, 1998·Journal of Biomechanics·H R ChaudhryN Guzelsu
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A M MalekS Izumo
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y J ShyyS Chien
Oct 13, 1992·Annals of the New York Academy of Sciences·M U NollertL V McIntire
May 5, 2012·PloS One·Prashanthi VandrangiVictor G J Rodgers
Aug 16, 2005·Experimental Dermatology·Heinfried H RadekeWolf-Henning Boehncke
Jul 25, 2009·Drug Metabolism Reviews·Katalin MonostoryZdenek Dvorak
Apr 29, 2008·Microvascular Research·Gavrielle M PriceJoe Tien
Nov 14, 2006·Life Sciences·H Greige-GergesN Ouaini
Jun 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J Y JeremyG D Angelini
Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y L Hu, S Chien
Oct 6, 2001·Critical Care Medicine·J A AdamsM A Sackner
Sep 1, 1991·Biotechnology and Bioengineering·M U NollertL V McIntire
Apr 1, 1995·Tissue Engineering·C W Patrick, L V McIntire
Mar 11, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·B W HochleitnerG Wick
Aug 13, 1999·The American Journal of Physiology·C HanW W Lautt
Mar 8, 2008·Applied Optics·J F FedericiA B Ritter
Nov 25, 2000·Journal of Applied Physiology·J A AdamsM A Sackner
Apr 1, 1997·The Journal of Surgical Research·R J SegurolaB E Sumpio
Jul 16, 1990·Biochemical and Biophysical Research Communications·M U NollertL V McIntire

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