The Effect of Short Nerve Grafts in Series on Axonal Regeneration Across Isografts or Acellular Nerve Allografts

The Journal of Hand Surgery
Ying YanAmy M Moore

Abstract

To evaluate the regenerative effect of the additional suture line when using either isografts (ISOs) or acellular nerve allografts (ANAs) placed end-to-end to span a short gap in a rat model. Rat sciatic nerves were transected and repaired with 2-cm nerve grafts (ISO or ANA). The grafts were 2 cm in length or a 1-cm segment was connected end-to-end to a 1-cm segment to yield a 2-cm length. At 8 weeks, extensor digitorum longus (EDL) muscle force and mass were measured. Nerves were harvested for histomorphometry. In a separate parallel study, the nerves were harvested 2 weeks following graft implantation to assess gene expression changes. All grafts demonstrated regeneration across the 2-cm segment(s). The additional suture line did not result in statistical differences in the number of myelinated nerve fibers that reached the distal nerve. However, when the graft types were compared, there was a significant decrease in nerve fibers in the ANA groups. The EDL muscle mass was significantly greater by using nerve ISOs compared with ANAs, regardless of an additional suture line, but there were no statistical differences noted in EDL muscle force. Gene expression analysis did not differ owing to an additional suture line. Minimal ax...Continue Reading

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Citations

Jul 12, 2018·Plastic and Reconstructive Surgery·Gwendolyn M HobenMatthew D Wood
Nov 14, 2019·The British Journal of Ophthalmology·Nate Jowett, Roberto Pineda Ii
Dec 5, 2018·Frontiers in Cellular Neuroscience·Arianna B LovatiStefania Raimondo
Dec 12, 2019·Frontiers in Bioengineering and Biotechnology·Cristiana R CarvalhoRui L Reis
Jun 15, 2020·Journal of Neurosurgery. Pediatrics·Laura G HamantJorge I Arango

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