PMID: 8603092Mar 13, 1996Paper

The effect of side-chain analogues of cholesterol on the thermotropic phase behavior of 1-stearoyl-2-oleoylphosphatidylcholine bilayers: a differential scanning calorimetric study

Biochimica Et Biophysica Acta
C VilchèzeRobert Bittman

Abstract

In this study we have examined the effects of analogues of cholesterol differing with respect to alkyl side-chain length and structure on the thermotropic phase behavior of bilayers formed from 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC), an important subclass of naturally occurring phosphatidylcholines (PCs). The synthetic sterols we studied contained either a terminally unbranched (n-series) or a single methyl-branched (iso-series) side chain of 3 to 10 carbon atoms. The phase transition behavior was examined by high-sensitivity differential scanning calorimetry (DSC). The main phase transition endotherm of SOPC/sterol bilayers consists of superimposed sharp and broad components, which represent the hydrocarbon chain melting of sterol-poor and sterol-rich phospholipid domains, respectively. The transition temperature and the cooperativity of the sharp component are moderately reduced upon sterol incorporation and the enthalpy decreases to zero when sterol levels of 20-30 mol% are reached. The enthalpy of the broad component transition initially increases to a maximum around 25 or 25-30 mol% sterol and thereafter decreases with further increases in sterol concentration. However, the broad transition of SOPC bilayers...Continue Reading

References

Jun 17, 1976·Biochimica Et Biophysica Acta·K E Suckling, G S Boyd
Oct 26, 1976·Biochimica Et Biophysica Acta·R A Demel, B De Kruyff
Apr 4, 1978·Biochimica Et Biophysica Acta·I F CraigK E Suckling
Dec 9, 1985·Biochimica Et Biophysica Acta·P L Yeagle
Dec 1, 1970·Biochimica Et Biophysica Acta·K W ButlerH Schneider
Dec 1, 1972·Biochimica Et Biophysica Acta·J C HsiaH D Gesser
Dec 16, 1974·Biochimica Et Biophysica Acta·J Goerke
Jan 17, 1972·Biochimica Et Biophysica Acta·R A DemelL L van Deenen
Dec 12, 1993·Biochimica Et Biophysica Acta·J Hernandez-Borrell, K M Keough

❮ Previous
Next ❯

Citations

Feb 25, 2003·Chemistry and Physics of Lipids·Richard M EpandShohei Maekawa
Oct 28, 2008·Journal of the American Chemical Society·Zhaohua Huang, Francis C Szoka
Dec 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Max LönnforsJ Peter Slotte
Sep 1, 2014·Chemistry and Physics of Lipids·Thomas MeyerHolger A Scheidt
Oct 1, 2011·Molecular Membrane Biology·Christoph StollWillem F Wolkers
Jan 3, 2006·Biophysical Journal·Ivan V Polozov, Klaus Gawrisch
Aug 20, 2005·Biochimica Et Biophysica Acta·Bernd W Koenig, Klaus Gawrisch
Sep 4, 2004·Biophysical Journal·Ivan V Polozov, Klaus Gawrisch
Apr 25, 2000·The Journal of Laboratory and Clinical Medicine·F TablinJ H Crowe
Apr 4, 2017·Chemistry and Physics of Lipids·Ole G MouritsenAdam Cohen Simonsen
Apr 21, 2017·Physical Chemistry Chemical Physics : PCCP·Julien MassiotVéronique Rosilio
Oct 2, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Jianjun PanJohn F Nagle
Jul 13, 2017·European Biophysics Journal : EBJ·Alexander L RabinovichDmitrii V Zhurkin
Nov 3, 2010·The Journal of Pharmacy and Pharmacology·M Habib AliYvonne Perrie
May 16, 1998·Biochimica Et Biophysica Acta·O P Bondar, E S Rowe
Aug 14, 1999·Biochimica Et Biophysica Acta·I V GrechishnikovaJ G Molotkovsky
Apr 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Paweł WydroMarta Lapczyńska
Feb 12, 2008·Biochimica Et Biophysica Acta·Alexander I GreenwoodStephanie Tristram-Nagle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.