Abstract
On a roundtrip to Mars, astronauts are expectedly exposed to an approximate amount of radiation that exceeds the lifetime limits on Earth. This elevated radiation dose is mainly due to Galactic Cosmic Rays and Solar Particle Events. Specific patterns of the N-glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing. The focus of our work was to investigate the effect of low-energy proton irradiation on the IgG N-glycosylation profile with the goal if disease associated changes could be detected during space travel and not altered by space radiation. Two ionization sources were used during the experiments, a Van de Graaff generator for the irradiation of solidified hIgG samples in vacuum, and a Tandetron accelerator to irradiate hIgG samples in aqueous solution form. Structural carbohydrate analysis was accomplished by CE with laser induced fluorescent detection to determine the effects of simulated space radiation on N-glycosylation of hIgG1 samples. Our results revealed that even several thousand times higher radiation doses that of astronauts can suffer during long duration missions beyond the shielding environment of ...Continue Reading
References
Feb 1, 1975·The British Journal of Radiology·H O Wyckoff
Apr 4, 1996·Nature·A Guttman
Oct 18, 2001·Radiation Research·F A CucinottaM Wear
Mar 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Christopher N ScanlanRaymond A Dwek
Jan 28, 2009·Nature Reviews. Microbiology·Michael J Daly
Aug 31, 2010·Experimental Gerontology·Valerie VanhoorenCuiying Chitty Chen
Feb 7, 2013·Methods in Molecular Biology·Chenchen WangCheng S Lee
Jun 1, 2013·Science·C ZeitlinG Reitz
Sep 25, 2014·Journal of the American Heart Association·Akintunde O AkinkuolieSamia Mora
Mar 3, 2015·Advances in Cancer Research·Naoyuki Taniguchi, Yasuhiko Kizuka
Aug 9, 2015·Analytical and Bioanalytical Chemistry·Peter A WillisMaria F Mora
Jun 28, 2016·Life Sciences in Space Research·Deepa M SridharanJanice M Pluth
Sep 21, 2016·Nature Communications·Takuma HashimotoTakekazu Kunieda
Oct 11, 2016·Scientific Reports·Vipan K PariharCharles L Limoli
Jan 25, 2017·Electrophoresis·Zsuzsanna KovacsAndras Guttman
May 4, 2017·Biochimica Et Biophysica Acta. General Subjects·Yasuhiko KizukaNaoyuki Taniguchi
May 31, 2017·Life Sciences in Space Research·Francis A CucinottaEliedonna Cacao
Mar 6, 2018·Radiation Research·Murat Alp, Francis A Cucinotta
Citations
Dec 22, 2020·International Journal of Radiation Biology·Yeni Natalia C Perez-GelvezCarl W Bergmann
May 28, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Antony LechnerYannis-Nicolas François
Sep 17, 2021·Electrophoresis·S Irem KayaSibel A Ozkan