The effect of sodium chloride on the structure of ribonucleoprotein particles from rat liver nuclei

Hoppe-Seyler's Zeitschrift für physiologische Chemie
W NorthemannP C Heinrich

Abstract

38S (monoparticles) and greater than 50--200S ribonucleoprotein particles (polyparticles) from rat liver nuclei were treated with increasing concentrations of sodium chloride. Treatment of 38S or greater than 50--200S particles, with 0.14, 0.25, 0.5, 1.0, and 2.0M NaCl resulted in a decrease of protein to RNA ratios from 8 to 3.1 for 38S particles and from 4.0 to 1.5 for greater than 20--200S particles. Correspondingly the densities in CsCl increased. Whereas the maximum of the sedimentation profile of polyparticles decreased from 90S to 50S after treatment with increasing NaCl concentrations, a discontinuous change was found in the case of monoparticles. It was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis that the proteins which were dissociated by NaCl were in the molecular weight range of 30--45 000. Four of the 5 small molecular weight RNAs in the range of 4.5 to 8S remained tightly associated even after treatment of polyparticles with 2.0M NaCl. When 38S or 70--200S nRNP particles were exposed to increasing concentrations of NaCl (0.25, 0.5, 1.0, 2.0M), the molar ellipticity at 264 nm increased progressively to about 40%. Upon NaCl treatment of polyparticles and successive removal of the dissociated p...Continue Reading

References

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Citations

May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J O ThomasW Szer
Sep 1, 1979·European Journal of Biochemistry·W NorthemannP C Heinrich
Apr 10, 1989·FEBS Letters·J Schenkel, P M Kloetzel
Nov 1, 1982·European Journal of Biochemistry·A PrüsseC E Sekeris

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