The effect of storage temperature and time on the concentrations of bovine serum amyloid a and its mammary associated isoform.

Veterinary Medicine International
Csilla TóthováGabriel Kováč

Abstract

The objective of this study was to evaluate the effect of storage under various conditions on the concentrations of major bovine acute phase protein-serum amyloid A, and its mammary isoform. Blood samples were taken from seven clinically healthy calves, and milk samples from six clinically healthy dairy cows. The harvested blood serum and the milk samples were fractioned into aliquots. One aliquot was analyzed on the day of collection without storage. The second aliquots were stored at 4°C for 1 day, the remaining aliquots were kept frozen at -18°C for 2, 7, 14, and 21 days, and then analyzed. Blood serum was analyzed for serum amyloid A (SAA). The concentrations of mammary isoform of SAA (M-SAA) were measured in milk samples. Over time, the concentrations of SAA in serum showed a tendency of significant decrease during storage at -18°C (P < 0.01). Similarly, the values of M-SAA decreased significantly in samples maintained at freezer temperatures (P < 0.001). In the refrigerated samples, we found non-significantly lower values of SAA, as well as M-SAA. Presented results indicate that the storage temperature and duration may markedly affect the concentrations of bovine SAA and M-SAA.

References

Mar 1, 1989·Equine Veterinary Journal·M B PepysP D Rossdale
Sep 8, 1998·The Biochemical Journal·L E Jensen, A S Whitehead
Nov 4, 1998·British Journal of Orthodontics·P A DowlingJ A Sandham
Oct 3, 1999·European Journal of Biochemistry·C M Uhlar, A S Whitehead
Feb 24, 2001·The Veterinary Record·P D EckersallJ L Fitzpatrick
Mar 29, 2001·Annals of Clinical Biochemistry·W W WoltersdorfA P Day
Dec 4, 2001·Veterinary Immunology and Immunopathology·T L McDonaldA Weber
Aug 21, 2003·Clinical Chemistry·Snjezana Rothkrantz-KosMarjolein Drent
May 26, 2004·The Veterinary Journal·H MurataM Yoshioka
Nov 24, 2004·Clinics in Laboratory Medicine·Ana K Stankovic
May 20, 2005·Veterinary Clinical Pathology·Jose Joaquýn CeronSilvia Martýnez-Subiela
Mar 11, 2006·Journal of Dairy Science·D M BarbanoM V Santos
Feb 16, 2008·Nephrology·Zoltan H Endre, Justin Westhuyzen
Sep 18, 2009·The Journal of Dairy Research·Maria AkerstedtAse Sternesjö
Feb 4, 2010·Acta Veterinaria Scandinavica·Anna HillströmInger Lilliehöök

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Citations

Sep 8, 2016·The Journal of Dairy Research·Funmilola C ThomasP David Eckersall
Oct 4, 2017·Acta Veterinaria Scandinavica·Michał CzopowiczJarosław Kaba
Jun 15, 2018·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Eva Maria GreunzMads Frost Bertelsen
Jun 11, 2021·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·E M TanvirP Nicholas Shaw

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Methods Mentioned

BETA
enzyme-linked
ELISA

Software Mentioned

GraphPad
GraphPad Prism

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