The effect of storage temperature on the biological activity of extracellular vesicles for the complement system

In Vitro Cellular & Developmental Biology. Animal
Sang June ParkMyung-Shin Lee

Abstract

Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.

References

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Citations

Dec 19, 2019·Tissue Engineering. Part B, Reviews·Richard JeskeYan Li
Apr 24, 2020·Journal of Cellular Physiology·Bo QinKun Xiong
Jun 20, 2020·Cellular Microbiology·Gabriele VargasLeonardo Nimrichter
Jan 14, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jun QiChunfeng Zhao
Dec 29, 2020·PloS One·Evgeniy G EvtushenkoElena Khomyakova
Feb 14, 2021·International Journal of Molecular Sciences·Aline Yen Ling Wang
Aug 20, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Laura CabezaJose Prados
May 18, 2021·Biotechnology Reports·Sevindzh K KletukhinaMarina O Gomzikova

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Methods Mentioned

BETA
electrophoresis
ELISA

Software Mentioned

ZetaView

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