The effect of substratum type, orientation and depth on the development of bacterial deep-sea biofilm communities grown on artificial substrata deployed in the Eastern Mediterranean

Biofouling
Nikoleta BellouFranciscus Colijn

Abstract

An increasing number of deep-sea studies have highlighted the importance of deep-sea biofouling, especially in relation to the protection of deep-sea instruments. In this study, the microbial communities developed on different substrata (titanium, aluminum, limestone, shale and neutrino telescope glass) exposed for 155 days at different depths (1500 m, 2500 m, 3500 m and 4500 m) and positions (vertical and horizontal) in the Eastern Mediterranean Deep Sea were compared. Replicated biofilm samples were analyzed using a Terminal Restriction Fragment Length Polymorphisms (T-RFLP) method. The restriction enzymes CfoI and RsaI produced similar total numbers (94, 93) of different T-RFLP peaks (T-RFs) along the vertical transect. In contrast, the mean total T-RF number between each sample according to substratum type and depth was higher in more samples when CfoI was used. The total species richness (S) of the bacterial communities differed significantly between the substrata, and depended on the orientation of each substratum within one depth and throughout the water column (ANOVA). T-RFLP analyses using the Jaccard similarity index showed that within one depth layer, the composition of microbial communities on different substrata wa...Continue Reading

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Citations

Aug 24, 2013·Biofouling·Alexandra MeierDouglas P Connelly
Dec 5, 2015·Frontiers in Microbiology·Elisa L-Y TanKatherine A Dafforn
Jul 23, 2013·Environmental Microbiology·Maria SaltaJean-Francois Briand
Sep 25, 2018·Environmental Microbiology Reports·Jorge AntunesVitor Vasconcelos
Jan 10, 2019·Biofouling·Sergey DobretsovSergey Piontkovski
Jan 7, 2019·Microbial Ecology·Wei DingPei-Yuan Qian
Dec 18, 2015·World Journal of Gastroenterology : WJG·Zhi-Mei XieXiao-Fang Pei

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Methods Mentioned

BETA
PCR
Scanning Electron Microscopy
electrophoresis
PCRs
restriction digests
MDS

Software Mentioned

TAP
PRIMER
GelQuest
NESTOR
ANTARES
GeneScan
TRFLP Java Applet
STATISTICA
TRFLP

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