The effect of sucrose on the rate of de novo sucrose biosynthesis in leaf protoplasts from spinach, wheat and barley

Archives of Biochemistry and Biophysics
C FoyerD Walker

Abstract

Protoplasts from the leaves of wheat, spinach, and barley were found to synthesize [14C]sucrose from 14CO2 at rates comparable with those of the parent tissue. CO2 fixation and sucrose biosynthesis ceased virtually immediately when the light was switched off. The effect of sucrose pretreatment on the rate of de novo sucrose biosynthesis was found to vary with leaf age and with plant species. Protoplasts from young wheat and spinach leaves showed an apparent stimulation of the rate of sucrose biosynthesis after sucrose pretreatment. In protoplasts from mature leaves of spinach, sucrose pretreatment produced inhibition. After sucrose pretreatment protoplasts from mature spinach leaves showed low rates of CO2 fixation, and sucrose biosynthesis compared with controls. Conversely, with protoplasts from mature leaves of wheat and barley, the rate of CO2 fixation was unchanged and there was little or no effect on the rate of sucrose biosynthesis after sucrose pretreatment. Preincubation with sucrose had no effect on the activity of sucrose-phosphate synthetase (EC 2.4.1.14), cytoplasmic fructose-1,6-bisphosphatase (EC 3.1.3.11), or UDPglucose pyrophosphorylase (EC 2.7.7.9) from spinach leaves. It was concluded that there is no direct ...Continue Reading

References

Dec 19, 1974·Biochimica Et Biophysica Acta·R M Lilley, D A Walker
Feb 1, 1967·The Biochemical Journal·J S Hawker
Apr 1, 1980·Archives of Biochemistry and Biophysics·R C Leegood, D A Walker

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Citations

May 28, 2013·Plant Physiology and Biochemistry : PPB·Qing-Lin LiuHai-Qing Zhang
Oct 7, 2011·Plant Biology·G Barba-EspínP Díaz-Vivancos

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