The Effect of Surface Modification of Aligned Poly-L-Lactic Acid Electrospun Fibers on Fiber Degradation and Neurite Extension

PloS One
Nicholas J SchaubRyan J Gilbert

Abstract

The surface of aligned, electrospun poly-L-lactic acid (PLLA) fibers was chemically modified to determine if surface chemistry and hydrophilicity could improve neurite extension from chick dorsal root ganglia. Specifically, diethylenetriamine (DTA, for amine functionalization), 2-(2-aminoethoxy)ethanol (AEO, for alcohol functionalization), or GRGDS (cell adhesion peptide) were covalently attached to the surface of electrospun fibers. Water contact angle measurements revealed that surface modification of electrospun fibers significantly improved fiber hydrophilicity compared to unmodified fibers (p < 0.05). Scanning electron microscopy (SEM) of fibers revealed that surface modification changed fiber topography modestly, with DTA modified fibers displaying the roughest surface structure. Degradation of chemically modified fibers revealed no change in fiber diameter in any group over a period of seven days. Unexpectedly, neurites from chick DRG were longest on fibers without surface modification (1651 ± 488 μm) and fibers containing GRGDS (1560 ± 107 μm). Fibers modified with oxygen plasma (1240 ± 143 μm) or DTA (1118 ± 82 μm) produced shorter neurites than the GRGDS or unmodified fibers, but were not statistically shorter than un...Continue Reading

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Citations

Dec 10, 2015·Journal of Neurotrauma·Nicholas J SchaubRyan J Gilbert
Jan 27, 2017·Neural Regeneration Research·Nicholas J Schaub
Jul 6, 2017·Pharmaceuticals·Angela FaccendiniFranca Ferrari
May 16, 2018·Biomedical Materials·Christopher D JohnsonRyan J Gilbert
Sep 6, 2019·International Journal of Molecular Sciences·Yevheniia NesterenkoOlga Mayans
Mar 7, 2021·Nanomaterials·Olga Y AntonovaYuri M Shlyapnikov

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Methods Mentioned

BETA
X-ray
chemical modification
chemical treatment
scanning electron microscopy
Scanning

Software Mentioned

ImageJ
JMP

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