PMID: 25791656Mar 21, 2015Paper

The effect of survivin expression on the apoptosis and proliferation of hypoxic human pulmonary arterial smooth muscle cells

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
Shuai ZhangZaiwen Fan

Abstract

Survivin is a new member of the inhibitor of apoptosis (IAP) family of proteins, which is also the strongest inhibitor of apoptosis.It inhibits apoptosis and promotes cell proliferation. This study aims to explore the effect of survivin expression on the apoptosis and proliferation of hypoxic human pulmonary arterial smooth muscle cells (HPASMCs). HPASMCs were divided randomly into 6 groups:Normoxia (N group):cultured under normoxia;Normoxia+YM155(NY group):cultured under normoxia with YM155, a selective survivin inhibitor, for 24 h;Hypoxia(H group):cultured under hypoxia for 24 h;Hypoxia+1 nmol/L YM155 (HY1 group):cultured under hypoxia with 1 nmol/L YM155 for 24 h; Hypoxia+10 nmol/L YM155 (HY10 group):cultured under hypoxia with 10 nmol/L YM155 for 24 h;Hypoxia+100 nmol/L YM155 (HY100 group):cultured under hypoxia with 100 nmol/L YM155 for 24 h. The mRNA and protein expressions of survivin were measured by real time PCR and Western Blot respectively. Cell proliferation was determined using a Cell Counting Kit-8 (CCK-8). Apoptosis was detected with a TUNEL test. The mRNA and protein expressions of survivin were observed in the H group (17 086 ± 1 044, 0.837 ± 0.027), but not in the N group (1, 0.016 ± 0.06) . The cell prolifer...Continue Reading

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Apoptosis

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