The effect of swainsonine on the phagocytosis of rod outer segments by rat RPE

Current Eye Research
D L Boyle, B J McLaughlin

Abstract

From studies using inhibitors such as tunicamycin and castanospermine, it has been suggested that plasma membrane glycoproteins may function as receptors in the phagocytosis of rod outer segments (ROS) by the retinal pigment epithelium (RPE). The exact structure of the oligosaccharide side chain of the glycoprotein may not be critical for this process. We have employed another inhibitor, swainsonine, which inhibits mannosidase II, a terminal enzyme in the protein glycosylation pathway, which results in membrane glycoproteins having hybrid-type oligosaccharide chains and fewer complex oligosaccharide chains. We have examined the ability of cultured rat RPE explants to phagocytize fluorescein isothiocyanate (FITC) labelled ROS or latex beads in the presence and absence of swainsonine. A significant (p less than 0.05) reduction in the phagocytosis of FITC-ROS was found between the swainsonine treated (37.7 +/- 4.1%) and untreated (85.4 +/- 2.7%) RPE explants. The nonspecific uptake of latex beads in both swainsonine treated (85.3 +/- 2.4%) and untreated (89.3 +/- 2.0%) RPE explants indicate that the RPE cells retained their ability to phagocytize. Major differences in spectrophotometric analysis of WGA-stained blots were an absenc...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 15, 1978·Science·A I Goldman, P J O'Brien
Aug 1, 1976·Experimental Eye Research·M FunahashiT Kuwabara
Jan 1, 1977·Methods in Enzymology·G Legler
Dec 1, 1987·Experimental Eye Research·M O Hall, T Abrams
Mar 16, 1988·Journal of Immunological Methods·D SamuelR A Abuknesha
Jun 24, 1985·Biochimica Et Biophysica Acta·U FuhrmannH Ploegh
May 1, 1988·Experimental Eye Research·J J Plantner, E L Kean
Sep 1, 1988·Archives of Ophthalmology·L V Del PrioreW R Green
Jul 1, 1986·The Journal of Cell Biology·P L Mayerson, M O Hall
Mar 1, 1987·Experimental Eye Research·B J McLaughlin, L G Boykins
Jan 1, 1984·CRC Critical Reviews in Biochemistry·A D Elbein
Jun 1, 1983·Journal of Cellular Physiology·A D ElbeinK Vosbeck
Oct 1, 1984·Experimental Cell Research·R Seyfried-WilliamsN G Cooper
Jun 1, 1984·Journal of Neurocytology·B J McLaughlinR B Caldwell
Feb 10, 1984·The Journal of Comparative Neurology·B J McLaughlin, L G Boykins
Jan 1, 1983·Vision Research·A R Heath, S F Basinger
Jan 1, 1983·Vision Research·R Seyfried-Williams, B J McLaughlin
Jul 1, 1981·Experimental Eye Research·N J Philp, M H Bernstein
Jul 1, 1962·The Journal of Cell Biology·J E DOWLING, R L SIDMAN

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.