The effect of temperature on apoptosis and adipogenesis on skeletal muscle satellite cells derived from different muscle types

Physiological Reports
Rachel L HardingS G Velleman

Abstract

Satellite cells are multipotential stem cells that mediate postnatal muscle growth and respond differently to temperature based upon aerobic versus anaerobic fiber-type origin. The objective of this study was to determine how temperatures below and above the control, 38°C, affect the fate of satellite cells isolated from the anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b. femoris). At all sampling times, p. major and b. femoris cells accumulated less lipid when incubated at low temperatures and more lipid at elevated temperatures compared to the control. Satellite cells isolated from the p. major were more sensitive to temperature as they accumulated more lipid at elevated temperatures compared to b. femoris cells. Expression of adipogenic genes, CCAAT/enhancer-binding protein β (C/EBPβ) and proliferator-activated receptor gamma (PPARγ) were different within satellite cells isolated from the p. major or b. femoris. At 72 h of proliferation, C/EBPβ expression increased with increasing temperature in both cell types, while PPARγ expression decreased with increasing temperature in p. major satellite cells. At 48 h of differentiation, both C/EBPβ and PPARγ expression increased in the p. major and decreased i...Continue Reading

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Citations

May 27, 2017·Scientific Reports·Dylan C SarverChristopher L Mendias
Oct 13, 2020·Frontiers in Physiology·Edgar Orlando Oviedo-RondónMichael J Wineland
Nov 6, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jiahui XuSandra G Velleman
Mar 13, 2021·Acta histochemica·Boonyarit KamkrathokYupaporn Chaiseha

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Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

CellSens Imaging
SAS
AdipoRed
Statistical Analysis System ( SAS
CellSens

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