The effect of temperature on the antimicrobial activity of Optisol-GS

Cornea
Rashmi KapurJoel Sugar

Abstract

To determine the survival of different bacteria inoculated in Optisol-GS at refrigerated storage temperature (6 degrees C) and after subsequent warming to room temperature (19-22 degrees C). Staphylococcus aureus, Enterococcus faecium, Streptococcus pneumoniae, and Pseudomonas aeruginosa were chosen from stock clinical isolates for inclusion in the study. The first group consisted of 12 Optisol-GS vials. The second group consisted of 12 Optisol-GS vials containing corneas inappropriate for transplantation according to the Eye Bank Association of America (EBAA) protocols. Each group was inoculated with 3 concentrations of approximately 10, 10, and 10 colony-forming units (CFU)/mL of each bacterial species and then refrigerated per EBAA protocol. After 48 hours of refrigeration, all vials were placed at room temperature (RT) and counts were performed at 48, 50 (2 hour RT), 54 (6 hour RT), 60 (12 hour RT), 72 (24 hour RT), and 96 (48 hour RT) hours. At 96 hours, the corneal tissue from 10 and 10 inocula were cultured. All samples underwent serial dilution, spiral plating on blood agar plates, and incubation at 35 degrees C. Viable colony counts were determined at 24 hours. Except for the 10 CFU/mL inocula of P. aeruginosa, all iso...Continue Reading

References

Jul 1, 1979·American Journal of Ophthalmology·H EscapiniH E Kaufman
Oct 1, 1979·Southern Medical Journal·K Mascarella, H D Cavanagh
Sep 1, 1975·The Journal of Antibiotics·L E Bryan, H M Van Den Elzen
May 1, 1992·Cornea·L S WeckbachJ L Staneck
Jun 1, 1991·Archives of Ophthalmology·H E KaufmanE D Varnell
Sep 1, 1991·Cornea·P L FarrellM D Trousdale
Sep 1, 1986·Archives of Ophthalmology·D D SaggauG D Roberts
May 1, 1988·The British Journal of Ophthalmology·K TamakiH E Kaufman
Jan 1, 1987·Cornea·W D Mathers, M A Lemp
Mar 1, 1985·The British Journal of Ophthalmology·M S InslerL A Wilson
May 1, 1984·American Journal of Ophthalmology·T G Poole, M S Insler
Jan 1, 1983·Ophthalmology·A S LeveilleH D Cavanagh
Oct 1, 1982·Archives of Ophthalmology·G J Pardos, M A Gallagher
Oct 15, 1993·American Journal of Ophthalmology·J H LassH K Soong
Mar 15, 1993·American Journal of Ophthalmology·D G HwangT M Smith
Mar 15, 1993·American Journal of Ophthalmology·P M KloessL A Wilson
Jun 1, 1997·Archives of Ophthalmology·S J WiffenW M Bourne
Oct 30, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ger P A Bongaerts, Leonie E Jansen
Feb 14, 2004·American Journal of Ophthalmology·Derek Y KunimotoIrving Raber

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Citations

May 17, 2007·International Ophthalmology·Elisabeth PelsIlse Claerhout
Aug 28, 2007·Cornea·Sohela S Hassan, Kirk R Wilhelmus
Nov 11, 2009·Current Eye Research·Davi Chen WuRichard Yudi Hida
Dec 20, 2018·The British Journal of Ophthalmology·Nicola LauStephen J Tuft
Jul 30, 2018·Cell and Tissue Banking·Apostolos LazaridisKonstantinos Droutsas

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