The effect of temporary meiotic attenuation on the in vitro maturation outcome of bovine oocytes

In Vitro Cellular & Developmental Biology. Animal
T FarghalyA Ahmady

Abstract

The aim of this study was to investigate the effect of delaying maturation by extended culture of immature bovine oocytes in prematuration medium (PMC) containing single maturation inhibitor on their meiotic resumption and embryonic development. Bovine immature oocytes were cultured in M199 containing 10 μM of either inhibitor (roscovitine, cilostamide, or forskolin) for either 72 or 120 h followed by up to 48 h in maturation media supplemented with 7.5 IU follicle-stimulating hormone (FSH)/luteinizing hormone (LH). Two control groups were used. In untreated control, immature oocytes were cultured in the same medium as the experimental group without any inhibitors. In the FSH/LH control group, oocytes were cultured directly in the maturation medium supplemented with FSH/LH up to 48 h. In vitro matured oocytes were then inseminated with frozen-thawed bull sperm. Fertilization, defined as two-cell division 48 h post-insemination, and blastocyst formation were recorded. Total maturation rate for the 72-h group was 73%, 70%, 66%, and 69% for roscovitine, forskolin, cilostamide, and FSH/LH control, respectively, with no significant difference indicating that inhibitors have no negative effect on the oocyte maturation rate. The total...Continue Reading

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Citations

Oct 27, 2016·Zygote : the Biology of Gametes and Early Embryos·F M C CaixetaM A N Dode
Feb 13, 2018·Reproduction in Domestic Animals = Zuchthygiene·S HuC Zhu
Jul 23, 2020·Reproduction, Fertility, and Development·Elizabeth S MetcalfThomas O Leary

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