The effect of the acrodermatitis enteropathica mutation on zinc uptake in human fibroblasts

Biological Trace Element Research
F Vazquez, A Grider

Abstract

The acrodermatitis enteropathica (AE) mutation affects intestinal zinc absorption. Our goal was to determine whether the AE mutation affects zinc uptake in human fibroblasts. Zinc uptake was determined during initial rates of uptake (10 min) following incubation in HEPES/saline buffer. Zinc uptake (from 0.25 to 1 microM) into normal fibroblasts was significantly greater than into the AE fibroblasts (p < 0.05). In order to identify factors that may alter cellular zinc uptake and be affected by the AE mutation, zinc uptake in the presence of albumin or bicarbonate was measured. Albumin restricted zinc uptake in both normal and AE fibroblasts, whereas bicarbonate stimulated zinc uptake in the normal fibroblasts. The effect of bicarbonate on zinc uptake in the AE fibroblasts was significantly reduced in both the Pronase-sensitive and Pronase-resistant compartments. Following loading of the fibroblasts with 1 microM zinc for 60 min, zinc efflux and retention were measured. The AE mutation did not affect zinc retention compared to normal fibroblasts. We conclude that the AE mutation affects both zinc binding to the cell surface and its translocation across the plasma membrane into the cell, possibly mediated through a defective anion...Continue Reading

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Citations

Aug 1, 1996·Biological Trace Element Research·A Grider, F Vazquez
Oct 14, 2008·Genes & Nutrition·M Leigh Ackland, Agnes Michalczyk
Feb 12, 1998·Biochimica Et Biophysica Acta·C Y ChangA Grider
Jun 3, 2015·Nature Communications·Dolores Pérez-SalaF Javier Cañada
Mar 8, 2005·Nutrition Journal·Michael F MouatArthur Grider
Dec 4, 2014·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Shakhenabat KasanaWolfgang Maret
Jun 13, 2003·The Journal of Biological Chemistry·Jodi Dufner-BeattieGlen K Andrews
Nov 13, 2003·The Journal of Biological Chemistry·Byung-Eun KimMichael J Petris
Nov 12, 2005·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Regina Fölster-Holst, Peter Höger
Feb 22, 2000·The Journal of Biological Chemistry·L A Gaither, D J Eide
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Kuljeet KaurShailendra K Saraf

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