PMID: 8584237Oct 20, 1995Paper

The effect of the angiotensin II (AT1A) receptor stably transfected into human neuroblastoma SH-SY5Y cells on noradrenaline release and changes in intracellular calcium

Neuroscience Letters
R L McDonaldP F Vaughan

Abstract

A stable cell line expressing the angiotensin II (AII) receptor has been obtained by transfecting the human neuroblastoma SH-SY5Y with the plasmid pCEP4 containing the entire coding region of the rat angiotensin AII receptor AT1A. Angiotensin II (AII; 1-100 nM) evokes the release of [3H]noradrenaline ([3H]NA) in this cell line. Pretreatment with 100 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) enhances the AII-evoked release of [3H]NA approximately two-fold. Removal of extracellular Ca2+ ([Ca2+]o) decreases 100 nM AII-evoked release of [3H]NA by over 50% both in the presence and absence of TPA. AII increases intracellular Ca2+ ([Ca2+]i) in this cell line which is consistent with the AT1A receptor being coupled to phospholipase C. Pretreatment with 100 nM TPA for 8 min attenuated the effect of AII on [Ca2+]i. The effects of AT1A receptor stimulation are therefore regulated differently in this cell line by activation of protein kinase C (PKC). Thus a useful cell line has been obtained from the human neuroblastoma SH-SY5Y in which to study at the molecular level the mechanism(s) by which AII regulates NA release.

References

Dec 1, 1991·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·L A SechiM Schambelan
Dec 1, 1991·Journal of Neurochemistry·N P MurphyJ G McCormack
Feb 1, 1991·Trends in Pharmacological Sciences·P B TimmermansW F Herblin
Jun 1, 1993·Journal of Neurochemistry·P F VaughanC Peers
Jul 5, 1994·Biochemical Pharmacology·R L McDonaldP F Vaughan
Oct 15, 1995·European Journal of Pharmacology·A J BalmforthS G Ball

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Citations

Mar 5, 1999·Molecular Neurobiology·P F VaughanC Peers
Jul 22, 1998·Progress in Neurobiology·H Majewski, L Iannazzo
Dec 20, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Alicia C ReidRandi B Silver

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