PMID: 6106504Oct 1, 1980Paper

The effect of the interdependence of protein and bile salt concentrations on the measurement of cerebroside sulphate sulphatase activity in human leucocyte and fibroblast extracts

Biochimica Et Biophysica Acta
A PoulosA C Pollard

Abstract

The previously observed differences in properties of human leucocyte and fibroblast cerebroside sulphate sulphatase (cerebroside-3-sulphate 3-sulphohydrolase, EC 3.1.6.8) measured in vitro have been found to be due to subtle differences in incubation conditions. Maximum enzyme activity was observed with either crude sodium taurocholate or with pure sodium taurodeoxycholate. The optimum bile salt concentration of the enzyme in leucocyte or fibroblast extracts, but not the pure ox liver enzyme, was critically dependent on protein concentration. At low concentrations of the latter (less than 0.1 mg/ml), maximum activity was observed at taurocholate concentrations less than 0.5 mg/ml; at protein concentrations greater than 0.20 mg/ml substantially more bile acid (more than 1.3 mg/ml) was required to stimulate maximum activity. Addition of Triton X-100 or bovine serum albumin to the incubation mixtures increased the optimum taurocholate concentration. The dependence of the bile salt optimum on protein concentration appears to be related to the binding of the lipid substrate to membranous protein present in the tissue extracts. Release of the bound lipid is effected either by increasing the bile salt concentration or by adding Triton...Continue Reading

References

Nov 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Poulos, K Beckman
Jul 2, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Poulos, K Beckman
Oct 2, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Poulos, K Beckman
Nov 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Poulos, A C Pollard
May 24, 1973·Biochimica Et Biophysica Acta·H RoelfzemaJ H Veerkamp
Aug 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A Snyder, R O Brady
Jan 1, 1972·Journal of Neurochemistry·A K PercyM M Kaback
Apr 1, 1970·Chemistry and Physics of Lipids·W Shankland

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