PMID: 9650071Jul 3, 1998Paper

The effect of the intersubunit disulfide bond on the structural and functional properties of the small heat shock protein Hsp25

International Journal of Biological Macromolecules
A V ZavialovM Gaestel

Abstract

The murine small heat shock protein Hsp25 carries a single cysteine residue in position 141 of its amino acid sequence. Interestingly, Hsp25 can exist within the cell as covalently bound dimer which is linked by an intermolecular disulfide bond between two monomers. Oxidative stress caused by treatment of the cells with diamide, arsenite, or hydrogen peroxide leads to an increase in Hsp25-dimerisation which can be blocked by simultaneous treatment with reducing agents. Recombinant Hsp25 was prepared in an oxidized dimeric (oxHsp25) and reduced monomeric (redHsp25) from. The two species were compared with regard to secondary structure, stability, oligomerization properties and their chaperone activity. It is demonstrated by CD measurements in the far UV region that there are no significant differences in the secondary structure and temperature- or pH-stability of oxHsp25 and redHsp25. However, according to CD measurements in the near UV region an increase in the asymmetry of the microenvironment of aromatic residues in oxHsp25 is observed. Furthermore, an increase in stability of the hydrophobic environment of the tryptophan residues mainly located in the N-terminal domain of the protein against urea denaturation is detected in ...Continue Reading

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Citations

Oct 18, 2011·Cell Stress & Chaperones·Evgeny V MymrikovNikolai B Gusev
May 5, 2009·The Journal of Biological Chemistry·David HayesPhilip Graceffa
Oct 17, 2002·Cell Stress & Chaperones·Nicole Mounier, André-Patrick Arrigo
Aug 12, 2010·Bioscience, Biotechnology, and Biochemistry·Tofazzal HossainYoichi Aso
Oct 19, 2011·Biochemistry Research International·Philip Graceffa
Mar 13, 2010·Journal of Cellular Biochemistry·Nagarekha PasupuletiRam H Nagaraj
Feb 13, 2018·Cell Stress & Chaperones·Bita MosaddeghBehnam Rasti
Sep 12, 2000·Nature Cell Biology·J M BrueyC Garrido
Oct 21, 2011·Physiological Reviews·Evgeny V MymrikovNikolai B Gusev
Feb 26, 2020·International Journal of Molecular Sciences·Jaakko SarparantaBjarne Udd
Apr 29, 2015·Expert Review of Proteomics·Evgeny V Mymrikov, Martin Haslbeck
Apr 2, 2002·The Journal of Biological Chemistry·Philip EatonMichael J Shattock
Mar 8, 2019·Nature Communications·T Reid AldersonAndrew J Baldwin
Feb 16, 2021·Chemical Society Reviews·Chenyan LvGuanghua Zhao
Feb 8, 2021·Bioorganic & Medicinal Chemistry·Leah N MakleyJason E Gestwicki
Oct 17, 1998·Biochimica Et Biophysica Acta·A V ZavialovV P Zav'yalov
Oct 27, 2004·Biochemical and Biophysical Research Communications·Ivan S ChernikNikolai B Gusev

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