Abstract
In traditional Chinese medicine, the cause of weak bones or bone loss is generally regarded as a result of kidney deficiency. Fructus Cnidii (FC), which is also known as She-Chuang-Zi, is a traditional herb that has been claimed to have kidney warming effects that invigorate Yang. In this study, we tried to determine the bone production-inducing effect of FC on osteoblastic cells in vitro using osthole, the main component of FC. Osteoblasts were isolated from neonatal Sprague-Dawley rat calvaria using the tissue piece culture method and treated with various concentrations of osthole ranging from 2.5 to 640 microg/mL, together with a blank control. Cell proliferation, alkaline phosphatase (ALP) activity, and bone nodules were measured. The cells were examined by hematoxylin-eosin staining, the Gomori Calcium-Cobalt method and immunofluorescent staining. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (or MTT) assay, ALP assay, and bone nodule results indicated significantly enhanced osteoblastic proliferation and differentiation at concentrations of osthole ranging from 40 to 320 microg/mL. Concentrations lower than 40 microg/ mL seemed less effective, and cytotoxicity to osteoblasts was observed at concentratio...Continue Reading
References
Oct 1, 1994·Bone and Mineral·A SabokbarN Rushton
Sep 1, 2000·Science·P DucyG Karsenty
Sep 1, 2000·Science·G A Rodan, T J Martin
Jun 26, 2002·Biological & Pharmaceutical Bulletin·Xiao Xia LiTeruhiko Matsumiya
Jun 26, 2002·Biological & Pharmaceutical Bulletin·Hideaki MatsudaMichinori Kubo
Apr 12, 2003·Nature Reviews. Drug Discovery·Carilee BergPeter Kirkpatrick
Jun 24, 2003·Biomaterials·G CiapettiA Giunti
Aug 23, 2003·Arthritis Research & Therapy·Nancy E Lane, Ariella Kelman
Jan 30, 2004·Planta medica·Ling-Ling YangChing-Chiung Wang
Jun 16, 2005·The Journal of Pharmacology and Experimental Therapeutics·Po-Lin KuoJiunn-Kae Chang
Dec 6, 2005·Fitoterapia·Sukh Mahendra SinghPratima Shrivastava
Jun 22, 2007·Clinical and Experimental Pharmacology & Physiology·Qiang XuTorao Ishida
Jul 4, 2007·Journal of Biomedical Materials Research. Part a·Guo-Chung DongChun-Hsu Yao
Sep 4, 2007·The British Journal of Nutrition·Yan ZhangMan-Sau Wong
Citations
Apr 15, 2014·The Journal of Surgical Research·Li-Qun MoJun Zhou
Aug 6, 2015·The American Journal of Chinese Medicine·Yi-Min LiLu-Ping Qin
Aug 8, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Zhong-Rong ZhangChun Wai Chan
May 17, 2015·Journal of Ethnopharmacology·Qin LiZhong-jie Liu
Mar 30, 2013·Biochemical and Biophysical Research Communications·Wenxin GengFulin Chen
Jun 15, 2016·Chinese Journal of Integrative Medicine·Wing-Sum SiuPing-Chung Leung
Jan 6, 2018·Nutrients·Yalda ShokoohiniaAnupam Bishayee
Jul 18, 2017·Electrophoresis·Zhao ChenDongmei Sun
Jan 25, 2012·Applied Biochemistry and Biotechnology·Arijit JanaPradeep Kumar Das Mohapatra
Mar 9, 2018·Endocrine Connections·Jeonghoon HaMin-Hee Kim
Apr 5, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Zhang-Jin ZhangGrainne M McAlonan
Jun 6, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Fei ZhouYing Xia
Feb 3, 2018·Journal of Cellular Biochemistry·Shaofeng YangZhenhua Luo
Aug 21, 2021·BioMed Research International·Wenyang WeiMengkai Zheng
Mar 27, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xinbing ZhuQimin Zhan