The effect of the major components of Fructus Cnidii on osteoblasts in vitro

Journal of Acupuncture and Meridian Studies
Wenping ZhangTorao Ishida

Abstract

In traditional Chinese medicine, the cause of weak bones or bone loss is generally regarded as a result of kidney deficiency. Fructus Cnidii (FC), which is also known as She-Chuang-Zi, is a traditional herb that has been claimed to have kidney warming effects that invigorate Yang. In this study, we tried to determine the bone production-inducing effect of FC on osteoblastic cells in vitro using osthole, the main component of FC. Osteoblasts were isolated from neonatal Sprague-Dawley rat calvaria using the tissue piece culture method and treated with various concentrations of osthole ranging from 2.5 to 640 microg/mL, together with a blank control. Cell proliferation, alkaline phosphatase (ALP) activity, and bone nodules were measured. The cells were examined by hematoxylin-eosin staining, the Gomori Calcium-Cobalt method and immunofluorescent staining. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (or MTT) assay, ALP assay, and bone nodule results indicated significantly enhanced osteoblastic proliferation and differentiation at concentrations of osthole ranging from 40 to 320 microg/mL. Concentrations lower than 40 microg/ mL seemed less effective, and cytotoxicity to osteoblasts was observed at concentratio...Continue Reading

References

Sep 1, 2000·Science·G A Rodan, T J Martin
Jun 26, 2002·Biological & Pharmaceutical Bulletin·Xiao Xia LiTeruhiko Matsumiya
Jun 26, 2002·Biological & Pharmaceutical Bulletin·Hideaki MatsudaMichinori Kubo
Apr 12, 2003·Nature Reviews. Drug Discovery·Carilee BergPeter Kirkpatrick
Aug 23, 2003·Arthritis Research & Therapy·Nancy E Lane, Ariella Kelman
Jan 30, 2004·Planta medica·Ling-Ling YangChing-Chiung Wang
Jul 4, 2007·Journal of Biomedical Materials Research. Part a·Guo-Chung DongChun-Hsu Yao

❮ Previous
Next ❯

Citations

Apr 15, 2014·The Journal of Surgical Research·Li-Qun MoJun Zhou
Aug 6, 2015·The American Journal of Chinese Medicine·Yi-Min LiLu-Ping Qin
Aug 8, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Zhong-Rong ZhangChun Wai Chan
May 17, 2015·Journal of Ethnopharmacology·Qin LiZhong-jie Liu
Mar 30, 2013·Biochemical and Biophysical Research Communications·Wenxin GengFulin Chen
Jun 15, 2016·Chinese Journal of Integrative Medicine·Wing-Sum SiuPing-Chung Leung
Jan 6, 2018·Nutrients·Yalda ShokoohiniaAnupam Bishayee
Jan 25, 2012·Applied Biochemistry and Biotechnology·Arijit JanaPradeep Kumar Das Mohapatra
Mar 9, 2018·Endocrine Connections·Jeonghoon HaMin-Hee Kim
Apr 5, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Zhang-Jin ZhangGrainne M McAlonan
Jun 6, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Fei ZhouYing Xia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.