The effect of the neuropeptide substance P on desensitization of ATP receptors of PC12 cells

British Journal of Pharmacology
L KhirougAndrea Nistri

Abstract

1. Patch clamp recording (whole cell configuration) was employed to investigate the modulatory action of substance P on inward currents elicited by adenosine 5'-triphosphate (ATP, focally applied via a pressure pipette) from phaechromocytoma (PC12) cells usually held at -70 mV. 2. Bath-applied substance P (0.2-20 microM) had no effect on baseline membrane current but reversibly reduced ATP peak currents in a concentration-dependent fashion. The depressant effect was not associated with a change in the ATP current reversal potential. 3. Equiamplitude peak responses induced by 50 microM or 5 mM ATP were differentially affected by substance P which preferentially reduced currents evoked by 5 mM ATP. In the presence of substance P a conditioning pulse of ATP evoked a stronger depression to subsequent test pulses of the same agonist. 4. Combined patch clamping and confocal laser imaging of intracellular Ca2+ ([Ca2+]i) of single PC12 cells showed that substance P (applied by a pressure pipette) per se had no effect on [Ca2+]i or current baseline, although it reduced the inward current and associated [Ca2+]i rise elicited by ATP. 5. These results are interpreted as due to facilitation by substance P of desensitization of ATP-gated P2x...Continue Reading

References

Jul 1, 1987·Journal of Neurochemistry·S M SimaskoG A Weiland
Jan 1, 1985·General Pharmacology·G Burnstock, C Kennedy
Jun 1, 1983·British Journal of Pharmacology·P R AdamsS W Jones
Feb 1, 1984·The Journal of Physiology·D E Clapham, E Neher
Apr 1, 1993·Physiological Reviews·M Otsuka, K Yoshioka
Aug 1, 1996·Current Opinion in Neurobiology·G Burnstock, J N Wood
Nov 1, 1996·British Journal of Pharmacology·R GiniatullinA Nistri
Apr 1, 1997·British Journal of Pharmacology·L KhirougA Nistri

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Citations

Oct 24, 1998·British Journal of Pharmacology·R Stoop, J M Quayle
Oct 12, 1999·The Journal of Physiology·F M SmithR D Murrell-Lagnado

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