The effect of the source pressure on the abundance of ions of noncovalent protein assemblies in an electrospray ionization orthogonal time-of-flight instrument

Rapid Communications in Mass Spectrometry : RCM
N TahallahAlbert J R Heck

Abstract

The effect of elevating the pressure in the interface region of an electrospray ionization orthogonal time-of-flight mass spectrometer on the ion intensity of different noncovalent protein assemblies has been investigated. Elevating the pressure in the interface region generally led to an enhanced detection of high m/z ions. The optimum pressure was found to be dependent on the m/z value of the ions. This pressure effect should be carefully addressed when relating ion abundance in the mass spectra to solution phase abundance of noncovalent protein assemblies.

References

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Citations

Dec 14, 2004·Chemistry : a European Journal·M Isabel CatalinaAlbert J R Heck
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Lee Chuin ChenKenzo Hiraoka
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Stephen V SciutoLars Konermann
Oct 13, 2011·Journal of the American Society for Mass Spectrometry·Lee Chuin ChenKenzo Hiraoka
Mar 1, 2005·Journal of the American Society for Mass Spectrometry·Hsin-Hung HuangChun-Hung Lin
May 26, 2005·Journal of the American Society for Mass Spectrometry·Joseph A LooIgor V Chernushevich
Aug 9, 2005·Journal of the American Society for Mass Spectrometry·Johan LengqvistWilliam J Griffiths
Sep 19, 2006·Journal of the American Society for Mass Spectrometry·Brian L Boys, Lars Konermann
Jun 29, 2007·Journal of the American Society for Mass Spectrometry·Greg M WaittJon D Williams
Dec 27, 2008·Journal of the American Society for Mass Spectrometry·Charlotte A ScarffJames H Scrivens
Nov 26, 2009·Journal of the American Society for Mass Spectrometry·Claudia BichRenato Zenobi
Dec 31, 2002·Current Opinion in Structural Biology·Frank Sobott, Carol V Robinson
Mar 26, 2009·Journal of the American Chemical Society·Md Joynal AbedinTrevor Douglas
Dec 14, 2011·Journal of Medicinal Chemistry·Hannah J MapleJohn Crosby
Jan 25, 2011·Nature Chemistry·Charlotte UetrechtAlbert J R Heck
Jul 5, 2008·Nature Protocols·Brandon T RuotoloCarol V Robinson
Apr 5, 2011·Nature Structural & Molecular Biology·Matthijs M JoreStan J J Brouns
Jul 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Charlotte UetrechtAlbert J R Heck
Apr 21, 2012·The Journal of Biological Chemistry·Yi-Chin C TsaiManajit Hayer-Hartl
Apr 17, 2009·The Journal of Biological Chemistry·Martijn W H PinkseAlbert J R Heck
Dec 20, 2011·Molecular & Cellular Proteomics : MCP·Florian StengelCarol V Robinson
Feb 10, 2012·Journal of the Royal Society, Interface·Gillian R Hilton, Justin L P Benesch
Apr 17, 2009·Plant Physiology·Nicole G H LeferinkWillem J H van Berkel
Jan 1, 2010·Future Medicinal Chemistry·Valerie Vivat HannahSarah Sanglier-Cianférani
Mar 15, 2014·Science·Christoph A DiebolderPaul W H I Parren
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Joost SnijderAlbert J R Heck
Apr 5, 2014·Journal of the American Society for Mass Spectrometry·Philip LösslAlbert J R Heck
Jul 20, 2012·Journal of the American Society for Mass Spectrometry·Basak KükrerAlbert J R Heck
Jun 5, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Toon H EversMaarten Merkx
Jul 12, 2014·Annual Review of Analytical Chemistry·Joost Snijder, Albert J R Heck
Dec 19, 2012·Biochimica Et Biophysica Acta·A KonijnenbergF Sobott
Sep 10, 2013·International Journal of Molecular Sciences·Ajda Taler-VerčičEva Zerovnik
Oct 4, 2002·Rapid Communications in Mass Spectrometry : RCM·Johan LengqvistJan Sjövall
Sep 1, 2014·Current Opinion in Biotechnology·Basri GülbakanRenato Zenobi
Feb 18, 2010·International Journal of Mass Spectrometry·Matthew R Schenauer, Julie A Leary
Apr 14, 2009·Journal of Molecular Biology·Daniël C de GeusJan Pieter Abrahams
May 2, 2008·Journal of Molecular Biology·Kristina LorenzenAlbert J R Heck

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