PMID: 11683518Oct 31, 2001Paper

The effect of the vasoactive intestinal polypeptide agonist Ro 25-1553 on induced tone in isolated human airways and pulmonary artery

Naunyn-Schmiedeberg's Archives of Pharmacology
D T SchmidtKlaus F Rabe

Abstract

Ro 25-1553 is a metabolically stable analogue of endogenous vasoactive intestinal polypeptide (VIP). This compound is a potent bronchodilator in vitro as well as in vivo. Moreover, Ro 25-1553 has been shown to be highly selective of the VPAC2 receptor. We assessed the effect of Ro 25-1553 on isolated human bronchi and pulmonary arteries in vitro. Macroscopically normal human airways and pulmonary arteries were obtained from patients undergoing surgery for lung cancer. The relaxing capability of Ro 25-1553 on bronchial and pulmonary artery tone was measured using standard techniques. Bronchial rings were pre-contracted with 0.1 mM histamine, and tone in pulmonary artery rings was induced with 10 microM PGF2alpha. Increasing concentrations of Ro 25-1553 within a range of 1 pM to 10 microM were added and isometric tension changes were recorded. Ro 25-1553 caused a concentration-dependent relaxation of airway and pulmonary artery preparations, with an EC50 of approximately 10 nM and a maximal relaxation of 70%-75% of the induced tone. The presence of VPAC2 receptors in the two tissues, though low in density, was confirmed by in situ hybridization, immunocytochemistry and ligand binding. These findings indicate that the VIP analogue...Continue Reading

Citations

Apr 12, 2011·Respiratory Research·Dongmei WuYong Kiel Sung
Mar 7, 2007·Clinics in Chest Medicine·Dermot S O'CallaghanSean P Gaine
Dec 19, 2006·Regulatory Peptides· Anaid ShahbazianWilhelm Mosgoeller
Jul 20, 2007·European Journal of Clinical Investigation·D DobererV Petkov
Feb 14, 2006·European Journal of Pharmacology·David A GronebergAxel Fischer
Aug 4, 2005·Expert Review of Cardiovascular Therapy·Israel Rubinstein
Jun 21, 2014·Journal of Applied Physiology·Motokazu KogaTakahisa Goto
Apr 9, 2015·Journal of Cardiovascular Pharmacology·Mofei WangHirohisa Toga
Nov 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Minlin XuJason X-J Yuan
Jun 1, 2021·Frontiers in Cell and Developmental Biology·Gandhi F Pavón-RomeroLuis M Terán

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