PMID: 8987527Feb 1, 1996Paper

The effect of thermal deactivation on the properties and processing characteristics of E. coli

Bioseparation
M A CollisAnton P J Middelberg

Abstract

Recombinant microorganisms are often employed to produce proteins of commercial significance. It is important to render such organisms non-viable before culture fluids are released from the fermenter. Thermal deactivation is a superficially attractive option because of its simplicity. The effects of such a thermal deactivation step at the end of a recombinant fermentation are reported in this study. In particular, the consequences of this treatment for down-stream process operations, namely homogenisation and centrifugation, are analysed and discussed. Homogenisation efficiency was adversely affected by a simple treatment whereby cells are raised to 65 degrees C from stationary phase. Cell debris size was also significantly increased. These changes could be partially explained by an increase in fractional peptidoglycan crosslinkage and a decrease in mean cell length. A simple process modification removed these detrimental effects. Specifically, the addition of 15 g of glucose 15 mins prior to thermal deactivation enhances downstream processing. Disruption efficiency is increased above that for stationary-phase cells and the resultant cell debris size is significantly reduced, theoretically aiding inclusion body purification. Th...Continue Reading

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.