The effect of three lipid emulsions differing in fatty acid composition on growth, apoptosis and cell cycle arrest in the HT-29 colorectal cancer cell line

Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition
Aleix Sala-VilaPhilip C Calder

Abstract

An in vitro study showed that a lipid emulsion containing fish oil (FO) slows the growth of colon cancer cells and enhances their sensitivity to 5-fluorouracil (FU). The aim was to confirm this finding and to compare such an emulsion with an alternative to lowered n-6 fatty acid exposure. We determined the number of viable cells, apoptosis and cell cycle distribution of HT-29 cells after exposure to one of three lipid emulsions. Cell cycle distribution was also assessed after treatment with lipid emulsions and FU. The lipid emulsion containing FO induced a significant growth inhibitory effect without changing the percentage of apoptotic cells. Exposure to the other lipid emulsions had no effect on growth and decreased apoptosis. Each lipid emulsion potentiated the S phase-halting effect of 1 and 10microM FU. This effect also occurred at 0.1microM FU when the cells were exposed to the FO containing lipid emulsion. A lipid emulsion containing FO has a growth inhibitory effect on a human colon adenocarcinoma cell line, an effect not due to the induction of apoptosis, and potentiated the S phase-halting effect of FU. Thus, an FO lipid emulsion may be of benefit in colorectal cancer.

References

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Citations

Sep 6, 2011·Critical Reviews in Food Science and Nutrition·Aleix Sala-Vila, Philip C Calder
Aug 21, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Antonella RosaAngela Maria Falchi
Jan 7, 2014·PloS One·Sylwia Kedracka-KrokKrystyna Urbanska
Jul 17, 2015·JPEN. Journal of Parenteral and Enteral Nutrition·Philip C Calder
Feb 20, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jun-Kai YanTian Zhang
Oct 19, 2017·The Proceedings of the Nutrition Society·P C Calder
Sep 7, 2013·International Journal of Oncology·Yao WuJide Wang

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