The effect of thyroid hormones in vitro and in vivo on hypothalamic neurotensin release and content

Endocrinology
M C Sheppard, K I Shennan

Abstract

Neurotensin (NT) is a hypothalamic peptide which inhibits TSH secretion when administered intraventricularly to the rat. We have studied the effects of TRH, TSH, T3, and rT3 on the release of immunoreactive NT (iNT) from incubations of freshly dissected sections of whole hypothalamic tissue removed from male rats. The effects of T4 or propylthiouracil administration in vivo on hypothalamic iNT content and release was examined as well. T3 (10(-7) M) stimulated iNT release in vitro [45.3 +/- (SE) 1.8 vs. 32.0 +/- 1.9 pg/hypothalamus 30 min-1, P less than 0.005] and a dose-dependent response was observed from 10(-9) M to 10(-7) M; rT3, TRH, and TSH were without effect. T4 administration in vivo caused a reduction in hypothalamic iNT content (5.77 +/- 0.24 vs. 6.57 +/- 0.37 ng/mg protein, P less than 0.05) whereas the induction of hypothyroidism with propylthiouracil and 131I resulted in an increase in iNT content (8.25 +/- 0.27 ng/mg protein, P less than 0.001). Basal release of hypothalamic iNT was similar in all three groups but 58 mM KCl-induced release from hypothyroid hypothalami was impaired. These results indicate a positive influence of T3 on hypothalamic iNT release and have led us to the hypothesis that T3 exerts a negat...Continue Reading

Citations

Sep 27, 2003·Life Sciences·Mona BoulesElliott Richelson
Oct 18, 2000·Regulatory Peptides·B M Tyler-McMahonE Richelson
Apr 18, 2003·Thyroid : Official Journal of the American Thyroid Association·Carmen C Pazos-MouraEgberto Gaspar de Moura
Jul 1, 1986·British Journal of Pharmacology·E C GriffithsP S Widdowson
Dec 23, 1985·Brain Research·S HojvatA M Lawrence
Apr 1, 1997·Frontiers in Neuroendocrinology·W H Rostène, M J Alexander
Jan 1, 1987·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I ReisertJ Lauder
Oct 1, 1993·Journal of Endocrinological Investigation·R Toni, R M Lechan

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