The effect of time-dependent deformation of viscoelastic hydrogels on myogenic induction and Rac1 activity in mesenchymal stem cells

Biomaterials
Andrew R CameronJustin J Cooper-White

Abstract

Cell behaviours within tissues are influenced by a broad array of physical and biochemical microenvironmental factors. Whilst 'stiffness' is a recognised physical property of substrates and tissue microenvironments that influences many cellular behaviours, tissues and their extracellular matrices are not purely rigid but 'viscoelastic' materials, composed of both rigid-like (elastic) and dissipative (viscous) elements. This viscoelasticity results in materials displaying increased deformation with time under the imposition of a defined force or stress, a phenomenon referred to as time-dependent deformation or 'creep'. Previously, we compared the behaviour of human mesenchymal stem cells (hMSCs) on hydrogels tailored to have a constant stiffness, but to display varying levels of creep in response to an applied force. Using polyacrylamide as a model material, we showed that on high-creep hydrogels (HCHs), hMSCs displayed increased proliferation, spread area and differentiation towards multiple lineages, compared to their purely stiff analogue, with a particular propensity for differentiation towards a smooth muscle cell (SMC) lineage. In this present study, we investigate the mechanisms behind this phenomenon and show that hMSCs ...Continue Reading

References

Nov 1, 1996·The Journal of Clinical Investigation·T YasudaR C Harris
Jun 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J ThybergU Hedin
Dec 31, 1997·The Journal of Cell Biology·K TakaishiY Takai
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
Jun 27, 2000·Biophysical Journal·C M LoY L Wang
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·F C YangD A Williams
Jan 26, 2002·Molecular and Cellular Biology·Jill ThybollKarl Tryggvason
Apr 11, 2002·Nature Cell Biology·Martin A Schwartz, Mark H Ginsberg
Jul 10, 2002·The Journal of Cell Biology·Akira KatsumiMartin Alexander Schwartz
Feb 25, 2003·Nature Cell Biology·Anjaruwee S NimnualDafna Bar-Sagi
Mar 5, 2003·Journal of Cell Science·Donald E Ingber
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Stephen G BallCay M Kielty
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Jul 4, 2006·Biochemical and Biophysical Research Communications·Anup K Kundu, Andrew J Putnam
Aug 10, 2006·Biology of the Cell·Arthur GanzBenoît Ladoux
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Jun 19, 2007·The Journal of Biological Chemistry·Anita WoodsFrank Beier
Sep 25, 2007·Nature Materials·Matthew J DalbyRichard O C Oreffo
Mar 25, 2008·Molecular Human Reproduction·M C FerreiraF M Reis
May 3, 2008·Cancer Research·Courtney M WilliamsJean E Schwarzbauer
Aug 30, 2008·American Journal of Physiology. Cell Physiology·Andrew S RowlandsJustin J Cooper-White
Oct 11, 2008·Journal of Cellular Physiology·Tae-Jin KimYingxiao Wang
Nov 28, 2008·American Journal of Physiology. Cell Physiology·Qingzhong XiaoQingbo Xu
Jan 29, 2009·Journal of Cell Science·Emily J ArnsdorfChristopher R Jacobs
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Benjamin GeigerAlexander D Bershadsky
Jun 2, 2009·Cellular Signalling·Jessica Rossol-AllisonTimothy A Fields
Sep 22, 2009·Current Biology : CB·Eric A KleinRichard K Assoian
Oct 9, 2009·Tissue Engineering. Part C, Methods·Jessica E FrithPaul G Genever
Nov 27, 2009·American Journal of Physiology. Cell Physiology·Padmanabhan P Pattabiraman, Ponugoti Vasantha Rao

❮ Previous
Next ❯

Citations

Dec 1, 2015·Nature Materials·Ovijit ChaudhuriDavid J Mooney
Dec 4, 2014·Biomacromolecules·Clementine PradalJustin J Cooper-White
Oct 7, 2015·Bioconjugate Chemistry·Christopher B RodellJason A Burdick
Mar 11, 2015·Journal of the American Geriatrics Society·Lisa GouldKenneth Schmader
Dec 10, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Lisa GouldKenneth Schmader
May 20, 2015·Advanced Materials·Huiyuan Wang, Sarah C Heilshorn
Sep 2, 2014·Progress in Biophysics and Molecular Biology·Sebastien G M UzelRoger D Kamm
Aug 15, 2015·Oxidative Medicine and Cellular Longevity·Sébastien SartYan Li
Oct 11, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Andrew W HolleAdam J Engler
Oct 25, 2016·International Journal of Pharmaceutics·Sandra Camarero-Espinosa, Justin Cooper-White
Nov 1, 2016·Journal of Biomedical Materials Research. Part a·Bethany Almeida, Anita Shukla
Jan 13, 2015·Stem Cells Translational Medicine·Steffen CossonJustin J Cooper-White
Mar 24, 2018·Scientific Reports·Daniel YuanAlexander A Spector
Jan 10, 2018·Advanced Healthcare Materials·Daniel A FoytEileen Gentleman
Apr 5, 2017·Biofabrication·Onur BasDietmar W Hutmacher
Aug 19, 2017·Chemical Society Reviews·Tobin E Brown, Kristi S Anseth
Dec 9, 2017·Annual Review of Biomedical Engineering·Christopher M Madl, Sarah C Heilshorn
Jun 9, 2018·Advanced Materials·Pingqiang CaiXiaodong Chen
Jan 10, 2016·Journal of Biomechanical Engineering·Matthew Ondeck, Adam Engler
Apr 20, 2016·Journal of Biomechanical Engineering·Burak OzcanCevat Erisken
Jan 19, 2018·Nature Communications·Jessica E FrithJustin J Cooper-White
Dec 10, 2019·Advanced Healthcare Materials·Marco CantiniManuel Salmeron-Sanchez
Sep 30, 2020·Expert Opinion on Drug Discovery·Carley OrtChristopher Moraes
Sep 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·David F HolmesKarl E Kadler
Oct 5, 2018·Nature Communications·Silvia A FerreiraEileen Gentleman
Mar 29, 2018·APL Bioengineering·Adi de la ZerdaSarah C Heilshorn
Apr 18, 2020·Science Advances·Zhu ChengMatthew J Paszek
Nov 22, 2019·Physiological Reviews·Paul A JanmeyCynthia A Reinhart-King
Aug 28, 2020·Nature·Ovijit ChaudhuriVivek B Shenoy
Dec 24, 2018·Biomaterials Science·Kamol DeyLuciana Sartore
May 2, 2019·Journal of Cell Science·Carlos Matellan, Armando E Del Río Hernández
Mar 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·J CarthewV X Truong
Apr 11, 2019·Regenerative Biomaterials·Cody O Crosby, Janet Zoldan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.