Abstract
To investigate the effect of total glucoside of paeony (TGP) on gut microbiota in NOD mice with Sjögren's syndrome (SS), using high-throughput sequencing of 16SrRNA gene. Twenty-four NOD mice were randomly assigned to 4 groups (n = 6 per group): sham group receiving deionized water (0.4 ml), hydroxychloroquin group receiving hydroxychloroquin (0.4 ml), TGP group receiving TGP (0.4 ml), and TGP + hydroxychloroquin group receiving 0.4 ml TGP and 0.4 ml hydroxychloroquin. Balb/c mice (n = 6) receiving 0.4 ml deionized water were used as a control group. After intragastric injection of drugs for 8 weeks, feces were collected for high-throughput sequencing of 16SrRNA gene. The sequencing of 16SrRNA gene resulted in 3686 OTUs, and 10 phyla and 69 genera were identified. Compared with the control group, the indices of Chao, Ace and Shannon in the other 4 groups were significantly lower (P < 0.05), and the Simpson index were significantly higher in the TGP, hydroxychloroquine, and sham groups (P < 0.05). Compared with the sham group, the indices of Chao, Ace and Shannon were significantly higher (P < 0.05), whereas the Simpson index was significantly lower (P < 0.05) in the TGP and TGP + hydroxychloroquine groups. At phylum level, Bact...Continue Reading
References
Feb 10, 2006·Clinical and Vaccine Immunology : CVI·C Maldonado Galdeano, G Perdigón
Jul 29, 2010·JAMA : the Journal of the American Medical Association·Manuel Ramos-CasalsXavier Bosch
Apr 19, 2011·Anaerobe·Eugenia BezirtzoglouGjalt W Welling
Jun 21, 2011·Frontiers in Pharmacology·Dong-Yi He, Sheng-Ming Dai
Mar 23, 2012·The ISME Journal·Clara Belzer, Willem M de Vos
May 3, 2012·Nature Reviews. Rheumatology·Manuel Ramos-CasalsAthanasios G Tzioufas
Jul 4, 2012·International Journal of Systematic and Evolutionary Microbiology·Julia DownesWilliam G Wade
Jan 26, 2013·Genome Biology·Falk HildebrandJeroen Raes
Feb 26, 2013·Nature Reviews. Microbiology·Felix Sommer, Fredrik Bäckhed
Sep 17, 2013·PloS One·Bhanu Priya GaneshMichael Blaut
Nov 19, 2013·Nucleic Acids Research·Aidan C Parte
Nov 20, 2013·Current Opinion in Rheumatology·Samuel B BruscaJose U Scher
May 7, 2014·Cellular Microbiology·Charisse Petersen, June L Round
Oct 2, 2014·MBio·Arancha HeviaAbelardo Margolles
Jul 28, 2015·Nature Medicine·Xuan ZhangJun Wang
Mar 22, 2016·Experimental and Therapeutic Medicine·Guolin WuGuoyou Yu
Jul 2, 2016·Journal of Korean Medical Science·Chang Ho YoonWon Ryang Wee
Aug 16, 2016·International Immunopharmacology·Yingbo ZhouXiaomei Li
Sep 18, 2017·Complementary Therapies in Medicine·Jing LuoQing-Wen Tao
Sep 29, 2017·Rheumatology·Elizabeth J PriceUNKNOWN British Society for Rheumatology Standards, Guideline and Audit Working Group
Jun 5, 2019·Chinese Journal of Integrative Medicine·Guo-Lin WuTian-Yi Li
Sep 21, 2019·Current Protocols in Pharmacology·Monika D ScuronPaul Smith
May 7, 2020·Journal of Ethnopharmacology·Huajuan JiangYao He