The effect of toxofilin on the structure and dynamics of monomeric actin

FEBS Letters
Lívia CzimbalekGábor Hild

Abstract

The effects of toxofilin (an actin binding protein of Toxoplasma gondii) on G-actin was studied with spectroscopy techniques. Fluorescence anisotropy measurements proved that G-actin and toxofilin interact with 2:1 stoichiometry. The affinity of toxofilin to actin was also determined with a fluorescence anisotropy assay. Fluorescence quenching experiments showed that the accessibility of the actin bound ε-ATP decreased in the presence of toxofilin. The results can be explained by the shift of the nucleotide binding cleft into a closed conformational state. Differential scanning calorimetry measurements revealed that actin monomers become thermodynamically more stable due to the binding of toxofilin.

References

Aug 15, 1988·European Journal of Biochemistry·M MossakowskaH Strzelecka-Gołaszewska
Nov 1, 1974·Analytical Biochemistry·T W Houk, K Ue
Jan 27, 1995·The Journal of Biological Chemistry·I PerelroizenD Pantaloni
May 21, 1999·The Journal of Biological Chemistry·L Blanchoin, T D Pollard
Jun 13, 2003·Molecular Biology of the Cell·Violaine DelormeIsabelle Tardieux
Jul 13, 2004·Trends in Cell Biology·Ville O PaavilainenPekka Lappalainen
Aug 16, 2006·Cellular and Molecular Life Sciences : CMLS·J S AllinghamI Rayment
Oct 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sung Haeng LeeRoberto Dominguez
Jul 22, 2008·The FEBS Journal·Dmitrii I LevitskyOlga P Nikolaeva
May 30, 2012·Journal of Cell Science·Violaine Delorme-WalkerIsabelle Tardieux
Jul 13, 2013·Biochimica Et Biophysica Acta·Roland KardosGábor Hild

❮ Previous
Next ❯

Citations

Apr 16, 2016·Biochimica Et Biophysica Acta·Veronika Takács-KollárGábor Hild
Oct 26, 2016·Journal of Photochemistry and Photobiology. B, Biology·Veronika Takács-KollárGábor Hild

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.

Related Papers

Zdravstveni vestnik
M VALENTINCIC
Bollettino dell'Istituto sieroterapico milanese
E CUBONI
Biologist : Journal of the Institute of Biology
J Timmis
© 2021 Meta ULC. All rights reserved