The effect of treatment of radicular dentin on colonization patterns of C. albicans

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
B Tugba TurkBilge Hakan Sen

Abstract

The aim of this study was to observe the colonization pattern of C. albicans on treated and untreated radicular dentin. Root sections of 10 human mandibular premolar teeth were longitudinally separated into halves. The 20 halves were separated into 2 groups and each half served as its own control. In Group 1, only gross pulpal remnants were removed with pliers. Root canal walls in the corresponding 102 halves (Group 2) were instrumented with Gates-Glidden burs and treated with sequential use of 15% EDTA solution for 3 minutes and 2.5% NaOCl solution for 3 minutes. Finally, all teeth were washed with distilled water. Each specimen was placed individually in each well of a 24-well cell culture plate. After the assembly was sterilized with ethylene-oxide, the root canal of each specimen was inoculated with 20 microL of C. albicans (1-1.5 x 10(6) cfu/mL) that was kept in place for 24 hours for initial attachment. Then, 2 mL of SDB was added to each well and the assembly was placed in an incubator at 37 degrees C for 10 days. Following the incubation period, the specimens were washed, fixed, dehydrated, and processed for scanning electron microscopy. C. albicans was present on the root canal surfaces of all specimens; however, the c...Continue Reading

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Citations

Sep 28, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Emel Olga OnayGizem Berk
Jul 21, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Andréa Cruz CâmaraAna Cristina Regis de Barros Correia
Feb 20, 2016·Brazilian Oral Research·Rachel Garcia de MirandaAna Paula Vieira Colombo
Jul 23, 2021·PloS One·Alice AlbertiLakshman Perera Samaranayake

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